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首页> 外文期刊>Life sciences >Cholinergic brush cells in the trachea mediate respiratory responses to quorum sensing molecules.
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Cholinergic brush cells in the trachea mediate respiratory responses to quorum sensing molecules.

机译：气管中的胆碱能刷细胞介导呼吸反应对法定感测分子。

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摘要

The airway epithelial surface is constantly exposed to inhaled environmental factors and pathogens. Bitter "tasting" bacterial products such as quorum sensing molecules (QSM) can be detected by solitary chemosensory cells of the upper respiratory tract. Recently, we have shown that tracheal brush cells are cholinergic chemosensory cells affecting the respiration upon stimulation with bitter substances. Here, we explore the hypothesis that tracheal brush cells are capable of detection of bacterial products such as QSM resulting in changes in respiration and in induction of local effects, e.g. regulation of mucociliary clearance.Functional analyses of respiration were performed in the trachea using a newly established model for investigation of respiration in spontaneously breathing anesthetized mice upon isolated tracheal stimulation. Influence of N-3-oxododecanoyl-homoserine lactone (3-OxoC(12)-HSL) on cilia-driven particle transport speed (PTS) in the airways was investigated in acutely excised and submerged mouse tracheae.3-OxoC(12)-HSL, a Pseudomonas aeruginosa quorum sensing autoinducer, caused a drop in the respiratory rate 2 min after the application at the mucosal surface. The 3-OxoC(12)-HSL-induced effect on respiration was abolished by inhibition of nicotinic receptors with mecamylamine and by removal of the respiratory epithelium. At the same concentration, 3-OxoC(12)-HSL enhanced significantly PTS on the mucosal surface.We conclude that cholinergic airway epithelial cells sense bacterial QSM in the airway lining fluid and communicate this to the CNS via ACh release and nicotinic stimulation of sensory neurons. In addition, QSM enhance PTS.
机译：气道上皮表面不断暴露于吸入的环境因素和病原体。苦涩的“品尝”诸如仲裁感测分子（QSM）的细菌产品可以通过上呼吸道的孤育化细胞来检测。最近，我们已经表明，气管刷细胞是在用苦物质刺激时影响呼吸的胆碱能化学感染细胞。在这里，我们探讨了气管刷细胞能够检测细菌产品，例如QSM的细菌产品，导致呼吸的变化和局部效应的变化，例如，如此。对粘膜渗透的函数分析在气管中使用新建立的模型进行了用于分离的气管刺激后自发呼吸麻醉小鼠的呼吸调查。在急性切除和浸没的小鼠气管3-氧气（12）中，研究了N-3-氧代癸酰（3-氧代（12）-HSL）对气道中纤毛驱动的颗粒输送速度（PTS）的影响。 HSL，一种假单胞菌铜绿假单胞菌Quorum感应的自动化器，在粘膜表面施用后2分钟导致呼吸速率下降。通过抑制与咪酰胺的烟碱受体和去除呼吸上皮，通过抑制荧光素受体并通过去除呼吸上皮进行3-氧代（12）-HSL诱导的效果。在相同的浓度下，3-氧代（12）-HSL在粘膜表面上显着增强。我们得出结论，胆碱能气道上皮细胞感测气道衬里流体中的细菌QSM，并通过ACH释放和感觉烟碱刺激将其与CNS传达给CNS。神经元。此外，QSM增强PTS。

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