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Characterization of the interface of binary mixed DOPC:DOPS liposomes in water: The impact of charge condensation

机译:二元混合DOPC界面的表征:水中的多孔脂质体:电荷冷凝的影响

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摘要

Solutions of liposomes composed of binary mixtures of anionic dioleoylphosphatidylserine (DOPS) and zwitterionic dioleoylphosphatidylcholine (DOPC) are investigated with label-free angle-resolved (AR) second harmonic scattering (SHS) and electrophoretic mobility measurements. The membrane surface potential is extracted from the AR-SHS response. The surface potential changes from 10 to 145 mV with varying DOPS content (from 0% to 100%) and levels off already at similar to 10% DOPS content. The zeta-potential shows the same trend but with a drastically lower saturation value (-44 mV). This difference is explained by the formation of a condensed layer of Na+ counterions around the outer leaflet of the liposome as predicted by charge condensation theories for polyelectrolyte systems. (C) 2017 Author(s).
机译:采用无标记的角度分辨(AR)第二谐波散射(SHS)和电泳迁移率测量,研究了由无阴离子二氧酰基磷磷苷(DOPA)和两性离子二酰二酰基磷脂酰磷胆碱(DOPC)组成的脂质体的溶液。 膜表面电位从AR-SHS响应中提取。 表面电位从10到145 mV变化,随着不同的多孔含量(从0%至100%),并且已经类似于10%的多孔含量。 Zeta-潜力显示相同的趋势,但饱和值急剧下降(-44 mV)。 通过在脂质体的外叶片周围形成的脂质体周围的凝结层的浓缩层来说解释了这种差异,以通过电力电解质系统的电荷缩合理论预测。 (c)2017年作者。

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