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首页> 外文期刊>Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices >Core-shell structured Ag@C for direct electrochemistry and hydrogen peroxide biosensor applications
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Core-shell structured Ag@C for direct electrochemistry and hydrogen peroxide biosensor applications

机译：核-壳结构的Ag @ C，用于直接电化学和过氧化氢生物传感器应用

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Ag@C core-shell nano-composites have been prepared by a simple one-step hydrothermal method and are further explored for protein immobilization and bio-sensing. The electrochemical behavior of immobilized horseradish peroxidase (HRP) on Ag@C modified indium-tin-oxide (ITO) electrode and its application as H_2O_2 sensor are investigated. Electrochemical and UV-vis spectroscopic measurements demonstrated that Ag@C nano-composites provide excellent matrixes for the adsorption of HRP and the entrapped HRP retains its bioactivities. It is found that on the HRP-Ag@C/ITO electrode, HRP exhibited a fast electron transfer process and good electrocatalytic reduction toward H_2O_2. Under optimum experimental conditions the biosensor linearly responds to H_2O_2 concentration in the range of 5.0×10~(-7)-1.4×10~(-4)M with a detection limit of 2.0×10~(-7)M (S/N=3). The apparent Michaelis-Menten constant (K _M~(app)) of the biosensor is calculated to be 3.75×10~(-5)M, suggesting high enzymatic activity and affinity toward H_2O_2. In addition, the HRP-Ag@C/ITO bio-electrode shows good reproducibility and long-term stability. Thus, the core-shell structured Ag@C is an attractive material for application in the fabrication of biosensors due to its direct electrochemistry and functionalized surface for efficient immobilization of bio-molecules.
机译：Ag @ C核壳纳米复合材料已通过简单的一步水热法制备，并进一步探索了蛋白质固定化和生物传感的方法。研究了辣根过氧化物酶（HRP）在Ag @ C修饰的氧化铟锡（ITO）电极上的电化学行为及其在H_2O_2传感器中的应用。电化学和紫外可见光谱测量表明，Ag @ C纳米复合材料为HRP的吸附提供了极好的基质，并且被捕获的HRP保留了其生物活性。发现在HRP-Ag @ C / ITO电极上，HRP表现出快速的电子转移过程和对H_2O_2的良好电催化还原。在最佳实验条件下，生物传感器对H_2O_2浓度在5.0×10〜（-7）-1.4×10〜（-4）M范围内线性响应，检出限为2.0×10〜（-7）M（S / N = 3）。该生物传感器的表观米氏（Michaelis-Menten）常数（K_M〜（app））经计算为3.75×10〜（-5）M，表明其高的酶活性和对H_2O_2的亲和力。此外，HRP-Ag @ C / ITO生物电极显示出良好的重现性和长期稳定性。因此，核壳结构的Ag @ C由于具有直接的电化学作用和功能化的表面，可以有效地固定生物分子，因此是用于制造生物传感器的一种有吸引力的材料。

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