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首页> 外文期刊>Journal of Molecular Biology >Revisit of Reconstituted 30-nm Nucleosome Arrays Reveals an Ensemble of Dynamic Structures
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Revisit of Reconstituted 30-nm Nucleosome Arrays Reveals an Ensemble of Dynamic Structures

机译：重构的30nm核小阵列的重新审查显示动态结构的集合

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摘要

It has long been suggested that chromatin may form a fiber with a diameter of similar to 30 nm that suppresses transcription. Despite nearly four decades of study, the structural nature of the 30-nm chromatin fiber and conclusive evidence of its existence in vivo remain elusive. The key support for the existence of specific 30-nm chromatin fiber structures is based on the determination of the structures of reconstituted nucleosome arrays using X-ray crystallography and single-particle cryo-electron microscopy coupled with glutaraldehyde chemical cross-linking. Here we report the characterization of these nucleosome arrays in solution using analytical ultracentrifugation, NMR, and small-angle X-ray scattering. We found that the physical properties of these nucleosome arrays in solution are not consistent with formation of just a few discrete structures of nucleosome arrays. In addition, we obtained a crystal of the nucleosome in complex with the globular domain of linker histone H5 that shows a new form of nucleosome packing and suggests a plausible alternative compact conformation for nucleosome arrays. Taken together, our results challenge the key evidence for the existence of a limited number of structures of reconstituted nucleosome arrays in solution by revealing that the reconstituted nucleosome arrays are actually best described as an ensemble of various conformations with a zigzagged arrangement of nucleosomes. Our finding has implications for understanding the structure and function of chromatin in vivo. Published by Elsevier Ltd.
机译：已经注意到，染色质可以形成直径与30nm的纤维抑制转录的纤维。尽管近四十年的研究，但30nm染色质纤维的结构性和其存在在体内存在的确凿证据仍然难以捉摸。特定30nm染色质纤维结构存在的关键支持基于使用X射线晶体学和单颗粒冷冻电子显微镜与戊二醛化学交联的单颗粒冷冻电子显微镜的重构核小体阵列的结构的测定。在这里，我们使用分析超速离心，NMR和小角X射线散射来报告这些核小组阵列的表征。我们发现，这些核心阵列的溶液中的物理性质与形成核心阵列的几个离散结构的形成不一致。此外，我们与链接组组蛋白H5的球状结构域的核心晶体中获得了核心的晶体，其显示了一种新的核小体包装形式，并表明核小阵列的可兼容替代紧凑型构象。我们的结果通过揭示重构的核小阵列实际上最好被描述为具有Z字形的核心排列的各种构象的集合，挑战溶液中存在有限数量的重构核心阵列的结构的关键证据。我们的发现对了解体内染色质的结构和功能有影响。 elsevier有限公司出版

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