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首页> 外文期刊>Journal of Neurophysiology >5-Hydroxytryptamine 2C receptors tonically augment synaptic currents in the nucleus tractus solitarii
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5-Hydroxytryptamine 2C receptors tonically augment synaptic currents in the nucleus tractus solitarii

机译：5-羟基羟基胺2C受体在核心菌中注射突触电流

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摘要

The nucleus tractus solitarii (nTS) is the primary termination and integration point for visceral afferents in the brain stem. Afferent glutamate release and its efficacy on postsynaptic activity within this nucleus are modulated by additional neuromodulators and transmitters, including serotonin (5-HT) acting through its receptors. The 5-HT_2 receptors in the medulla modulate the cardiorespiratory system and autonomic reflexes, but the distribution of the 5-HT_(2C) receptor and the role of these receptors during synaptic transmission in the nTS remain largely unknown. In the present study, we examined the distribution of 5-HT_(2C) receptors in the nTS and their role in modulating excitatory postsynaptic currents (EPSCs) in monosynaptic nTS neurons in the horizontal brain stem slice. Real-time RT-PCR and immunohisto-chemistry identified 5-HT_(2C) receptor message and protein in the nTS and suggested postsynaptic localization. In nTS neurons innervated by general visceral afferents, 5-HT_(2C) receptor activation increased solitary tract (TS)-EPSC amplitude and input resistance and depolarized membrane potential. Conversely, 5-HT_(2C) receptor blockade reduced TS-EPSC and miniature EPSC amplitude, as well as input resistance, and hyperpolarized membrane potential. Synaptic parameters in nTS neurons that receive sensory input from carotid body chemoafferents were also attenuated by 5-HT_(2C) receptor blockade. Taken together, these data suggest that 5-HT_(2C) receptors in the nTS are located postsynaptically and augment excitatory neurotransmission.
机译：核泌尿菌（NTS）是脑干中内脏引义的主要终端和整合点。传入谷氨酸释放及其对该细胞核内的突触后活性的疗效由另外的神经调节剂和发射器调节，包括通过其受体作用的血清素（5-HT）。髓质中的5-HT_2受体调节心肺系统和自主反应，但5-HT_（2C）受体的分布和这些受体在NTS中突触传递期间的作用仍然未知。在本研究中，我们研究了NTS中的5-HT_（2C）受体的分布及其在水平脑干切片中调节单腹部NT神经元中的兴奋性突触性电流（EPSC）的作用。实时RT-PCR和免疫组化学鉴定了NTS中的5-HT_（2C）受体消息和蛋白质，并提出了突触后定位。在通过通用内脏引入的NTS神经元中，5-HT_（2C）受体激活增加了孤立的躯干（TS）-EPSC振幅和输入电阻和去极化膜电位。相反，5-HT_（2C）受体封锁减少了TS-EPSC和微型EPSC振幅，以及输入电阻和超极化膜电位。 NTS神经元中接收来自颈动脉体培养件的感觉输入的NTS神经元的突触参数也衰减了5-HT_（2C）受体阻滞。总之，这些数据表明，NTS中的5-HT_（2C）受体突出地定位和增强兴奋性神经递质。

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