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首页> 外文期刊>Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis >Isotope-coded ESI-enhancing derivatization reagents for differential analysis, quantification and profiling of metabolites in biological samples by LC/MS: A review
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Isotope-coded ESI-enhancing derivatization reagents for differential analysis, quantification and profiling of metabolites in biological samples by LC/MS: A review

机译：同位素编码的ESI增强衍生化试剂，用于LC / MS在生物样本中的代谢产物的差异分析，定量和分析：综述

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摘要

The analysis of the qualitative and quantitative changes of metabolites in body fluids and tissues yields valuable information for the diagnosis, pathological analysis and treatment of many diseases. Recently, liquid chromatography/electrospray ionization-(tandem) mass spectrometry [LC/ESI-MS(/MS)] has been widely used for these purposes due to the high separation capability of LC, broad coverage of ESI for various compounds and high specificity of MS(/MS). However, there are still two major problems to be solved regarding the biological sample analysis; lack of sensitivity and limited availability of stable isotope-labeled analogues (internal standards, ISs) for most metabolites. Stable isotope-coded derivatization (ICD) can be the answer for these problems. By the ICD, different isotope-coded moieties are introduced to the metabolites and one of the resulting derivatives can serve as the IS, which minimize the matrix effects. Furthermore, the derivatization can improve the ESI efficiency, fragmentation property in the MS/MS and chromatographic behavior of the metabolites, which lead to a high sensitivity and specificity in the various detection modes. Based on this background, this article reviews the recently reported isotope-coded ESI-enhancing derivatization (ICEED) reagents, which are key components for the ICD-based LC/MS(/MS) studies, and their applications to the detection, identification, quantification and profiling of metabolites in human and animal samples. The LC/MS(/MS) using the ICEED reagents is the powerful method especially for the differential analysis (relative quantification) of metabolites in two comparative samples, simultaneous quantification of multiple metabolites whose stable isotope-labeled ISs are not available, and submetabolome profiling. (C) 2016 Elsevier B.V. All rights reserved.
机译：对体液和组织代谢物的定性和定量变化的分析产生了许多疾病的诊断，病理分析和治疗的有价值的信息。近来，液相色谱/电喷雾电离 - （串联）质谱[LC / ESI-MS）]由于LC的高分离能力，对于各种化合物和高特异性而广泛地用于这些目的。对于各种化合物和高特异性，ESI的广泛覆盖MS（/ MS）。然而，关于生物样本分析仍有两个主要问题;缺乏敏感性和稳定同位素标记的类似物（内标，ISS）的可用性有限的可用性。稳定的同位素编码衍生化（ICD）可以是这些问题的答案。通过ICD，将不同的同位素编码的部分引入代谢物，其中一个得到的衍生物可以用作最小化基质效应。此外，衍生化可以提高代谢物的MS / MS中的抗抗E效率，碎裂性质和代谢物的色谱行为，这导致各种检测模式中的高灵敏度和特异性。基于此背景，本文综述了最近报告的同位素编码的ESI增强衍生化（ICEED）试剂，其是基于ICD的LC / MS（/ MS）研究的关键组成部分，以及它们在检测，识别，识别的应用程序的关键组件，人畜样品代谢物的量化和分析。使用冰氧代谢的LC / MS（/ MS）是特别是对于两种比较样本中代谢物的差分分析（相对定量），同时定量稳定同位素标记的ISS的多种代谢物的差异定量，以及IMBETABOLOME分析。 （c）2016 Elsevier B.v.保留所有权利。

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