• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >The calcium silicate/alginate composite: Preparation and evaluation of its behavior as bioactive injectable hydrogels
【24h】

The calcium silicate/alginate composite: Preparation and evaluation of its behavior as bioactive injectable hydrogels

机译：硅酸钙/藻酸盐复合物：具有生物活性的可注射水凝胶的制备和性能评估

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this study, an injectable calcium silicate (CS)/sodium alginate (SA) hybrid hydrogel was prepared using a novel material composition design. CS was incorporated into an alginate solution and internal in situ gelling was induced by the calcium ions directly released from CS with the addition of d-gluconic acid δ-lactone (GDL). The gelling time could be controlled, from about 30 s to 10 min, by varying the amounts of CS and GDL added. The mechanical properties of the hydrogels with different amounts of CS and GDL were systematically analyzed. The compressive strength of 5% CS/SA hydrogels was higher than that of 10% CS/SA for the same amount of GDL. The swelling behaviors of 5% CS/SA hydrogels with different contents of GDL were therefore investigated. The swelling ratios of the hydrogels decreased with increasing GDL, and 5% CS/SA hydrogel with 1% GDL swelled by only less than 5%. Scanning electron microscopy (SEM) observation of the scaffolds showed an optimal interconnected porous structure, with the pore size ranging between 50 and 200 μm. Fourier transform infrared spectroscopy and SEM showed that the CS/SA composite hydrogel induced the formation of hydroxyapatite on the surface of the materials in simulated body fluid. In addition, rat bone mesenchymal stem cells (rtBMSCs) cultured in the presence of hydrogels and their ionic extracts were able to maintain the viability and proliferation. Furthermore, the CS/SA composite hydrogel and its ionic extracts stimulated rtBMSCs to produce alkaline phosphatase, and its ionic extracts could also promote angiogenesis of human umbilical vein endothelial cells. Overall, all these results indicate that the CS/SA composite hydrogel efficiently supported the adhesion, proliferation and differentiation of osteogenic and angiogenic cells. Together with its porous three-dimensional structure and injectable properties, CS/SA composite hydrogel possesses great potential for bone regeneration and tissue engineering applications.
机译：在这项研究中，使用新颖的材料组成设计制备了可注射的硅酸钙（CS）/海藻酸钠（SA）混合水凝胶。将CS掺入藻酸盐溶液中，并通过添加d-葡萄糖酸δ-内酯（GDL）从CS直接释放的钙离子诱导内部原位胶凝。通过改变CS和GDL的添加量，可以将胶凝时间控制在约30秒至10分钟。系统分析了CS和GDL含量不同的水凝胶的力学性能。对于相同量的GDL，5％CS / SA水凝胶的抗压强度高于10％CS / SA的抗压强度。因此研究了具有不同GDL含量的5％CS / SA水凝胶的溶胀行为。水凝胶的溶胀率随GDL的增加而降低，而1％GDL的5％CS / SA水凝胶仅溶胀不到5％。支架的扫描电子显微镜（SEM）观察显示了最佳的互连多孔结构，孔径范围为50至200μm。傅里叶变换红外光谱和SEM结果表明，CS / SA复合水凝胶在模拟体液中诱导了材料表面羟基磷灰石的形成。此外，在水凝胶及其离子提取物的存在下培养的大鼠骨间充质干细胞（rtBMSC）能够维持活力和增殖。此外，CS / SA复合水凝胶及其离子提取物刺激rtBMSCs产生碱性磷酸酶，其离子提取物也可促进人脐静脉内皮细胞的血管生成。总的来说，所有这些结果表明，CS / SA复合水凝胶有效地支持了成骨细胞和血管生成细胞的粘附，增殖和分化。 CS / SA复合水凝胶具有多孔的三维结构和可注射特性，在骨骼再生和组织工程应用中具有巨大的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号