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Protein thermostability engineering

机译：蛋白质热稳定性工程

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摘要

The use of enzymes for industrial and biomedical applications is limited to their function at elevated temperatures. The principles of thermostability engineering need to be implemented for proteins with low thermal stability to broaden their applications. Therefore, understanding the thermal stability modulating factors of proteins is necessary for engineering their thermostability. In this review, first different thermostability enhancing strategies in both the sequence and structure levels, discovered by studying the natural proteins adapted to different conditions, are introduced. Next, the progress in the development of various computational methods to engineer thermostability of proteins by learning from nature and introducing several popular tools and algorithms for protein thermostability engineering is highlighted. Further discussion includes the challenges in the field of protein thermostability engineering such as the protein stability- activity trade- off. Finally, how thermostability engineering could be instrumental for the design of protein drugs for biomedical applications is demonstrated.
机译：用于工业和生物医学应用的酶的使用限于它们在升高的温度下的功能。需要为具有低热稳定性的蛋白质来实施热稳定性工程的原理，以拓宽其应用。因此，理解蛋白质的热稳定性调节因子是工程的热稳定性所必需的。在本文中，介绍了通过研究适应于不同条件的天然蛋白质来发现序列和结构水平的第一种不同的热稳定性增强策略。接下来，通过从自然学习和引入蛋白质热能工程工程的几种流行工具和算法来开发蛋白质的各种计算方法的进展。进一步的讨论包括蛋白质热能性工程领域的挑战，例如蛋白质稳定性 - 活动折衷。最后，恒温性工程如何对生物医学应用的蛋白质药物设计有用。

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  • 来源
    《RSC Advances》 |2016年第116期|共19页
  • 作者

    Modarres H. Pezeshgi; Mofrad M. R.; Sanati-Nezhad A.;

  • 作者单位

    Univ Calif Berkeley Mol Cell Biomech Lab Dept Bioengn 208A Stanley Hall Berkeley CA 94720 USA;

    Univ Calif Berkeley Mol Cell Biomech Lab Dept Bioengn 208A Stanley Hall Berkeley CA 94720 USA;

    Univ Calgary Dept Mech &

    Mfg Engn BioMEMS &

    Bioinspired Microfluid Lab 2500 Univ Dr NW Calgary AB T2N 1N4 Canada;

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  • 正文语种 eng
  • 中图分类 化学;
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