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首页> 外文期刊>Acta biomaterialia >Multifunctional role of osteopontin in directing intrafibrillar mineralization of collagen and activation of osteoclasts
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Multifunctional role of osteopontin in directing intrafibrillar mineralization of collagen and activation of osteoclasts

机译：骨桥蛋白在指导胶原纤维内矿化和破骨细胞活化中的多功能作用

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Mineralized collagen composites are of interest because they have the potential to provide a bone-like scaffold that stimulates the natural processes of resorption and remodeling. Working towards this goal, our group has previously shown that the nanostructure of bone can be reproduced using a polymer-induced liquid-precursor (PILP) process, which enables intrafibrillar mineralization of collagen with hydroxyapatite to be achieved. This prior work used polyaspartic acid (pASP), a simple mimic for acidic non-collagenous proteins, to generate nanodropletsanoparticles of an amorphous mineral precursor which can infiltrate the interstices of type-I collagen fibrils. In this study we show that osteopontin (OPN) can similarly serve as a process-directing agent for the intrafibrillar mineralization of collagen, even though OPN is generally considered a mineralization inhibitor. We also found that inclusion of OPN in the mineralization process promotes the interaction of mouse marrow-derived osteoclasts with PILP-remineralized bone that was previously demineralized, as measured by actin ring formation. While osteoclast activation occurred when pASP was used as the process-directing agent, using OPN resulted in a dramatic effect on osteoclast activation, presumably because of the inherent arginine-glycine-aspartate acid ligands of OPN. By capitalizing on the multifunctionality of OPN, these studies may lead the way to producing biomimetic bone substitutes with the capability of tailorable bioresorption rates.
机译：矿化的胶原蛋白复合物是令人感兴趣的，因为它们有潜力提供刺激吸收和重塑的自然过程的骨样支架。朝着这个目标努力，我们的研究小组先前已经表明，可以使用聚合物诱导的液体前体（PILP）工艺复制骨骼的纳米结构，该工艺可以实现胶原纤维内的羟基磷灰石矿化作用。这项先前的工作使用了聚天冬氨酸（pASP）（一种酸性非胶原蛋白的简单模拟物）来生成无定形矿物前体的纳米滴/纳米颗粒，该颗粒可以渗入I型胶原原纤维的间隙中。在这项研究中，我们表明骨桥蛋白（OPN）可以类似地用作胶原纤维内矿化的过程指导剂，即使OPN通常被认为是矿化抑制剂。我们还发现，在矿化过程中加入OPN可以促进小鼠骨髓来源的破骨细胞与PILP矿化骨的相互作用，该骨先前已矿化，通过肌动蛋白环形成来衡量。当将pASP用作过程指导剂时发生破骨细胞活化，而使用OPN则对破骨细胞活化产生了显着影响，这可能是由于OPN固有的精氨酸-甘氨酸-天冬氨酸配体所致。通过利用OPN的多功能性，这些研究可能会引导生产具有可调节的生物吸收速率的仿生骨替代物。

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