...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochimica et biophysica acta: BBA: International journal of biochemistry, biophysics and molecular biololgy. Proteins and Proteomics >A novel beta-glucosidase isolated from the microbial metagenome of Lake Poraque (Amazon, Brazil)
【24h】

A novel beta-glucosidase isolated from the microbial metagenome of Lake Poraque (Amazon, Brazil)

机译：从湖泊湖（亚马逊，巴西）分离的新型β-葡糖苷酶（亚马逊）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Amazon region holds most of the biological richness of Brazil. Despite their ecological and biotechnological importance, studies related to microorganisms from this region are limited. Metagenomics leads to exciting discoveries, mainly regarding non-cultivable microorganisms. Herein, we report the discovery of a novel beta-glucosidase (glycoside hydrolase family 1) gene from a metagenome from Lake Poraque in the Amazon region. The gene encodes a protein of 52.9 kDa, named AmBgl-LP, which was recombinantly expressed in Escherichia soli and biochemically and structurally characterized. Although AmBgl-LP hydrolyzed the synthetic substrate pnitrophenyl-beta-D-glucopyranoside (pNP beta G) and the natural substrate cellobiose, it showed higher specificity for pNP beta G (k(cat)/K-m = 6 s(-1).mM(-1)) than cellobiose (k(cat)/K-m = 0.6 s(-1).mM(-1)) AmBgl-LP showed maximum activity at 40 degrees C and pH 6.0 when pNP beta G was used as the substrate. Glucose is a competitive inhibitor of AmBgl-LP, presenting a K-i of 14 mM. X-ray crystallography and Small Angle X-ray Scattering were used to determine the AmBgl-LP three-dimensional structure and its oligomeric state. Interestingly, despite sharing similar active site architecture with other structurally characterized GH1 family members which are monomeric, AmBgl-LP forms stable dimers in solution. The identification of new GH1 members by metagenomics might extend our understanding of the molecular mechanisms and diversity of these enzymes, besides enabling us to survey their industrial applications.
机译：亚马逊地区拥有大部分巴西的生物丰富性。尽管其生态和生物技术重要性，但与该地区的微生物相关的研究是有限的。 Metagenomics导致令人兴奋的发现，主要是关于不可种植的微生物。在此，我们报告了从亚马逊地区的波拉克湖的梅塔蛋白酶中发现了一种新的β-葡萄糖苷酶（糖苷水解酶1）基因。该基因编码一个名为AmbGl-LP的52.9kDa的蛋白质，该蛋白质在大肠杆菌溶液和生物化学和结构表征中重组。虽然AmbGl-LP水解了合成底物脱硝基苯β-D-吡喃葡萄糖苷（PNPβG）和天然底物纤维二糖，但它表明PNPβG（K（猫）/ km = 6s（-1）.mm的比例更高（-1））比纤维二糖（K（猫）/ km = 0.6 s（-1）.mm（-1））Ambgl-LP在使用PNPβG作为基材时显示40℃和pH 6.0的最大活性。葡萄糖是AMBGL-LP的竞争性抑制剂，呈现14毫米的K-1。使用X射线晶体学和小角度X射线散射来确定AmbGl-LP三维结构及其低聚状态。有趣的是，尽管与作为单体的其他结构特征的GH1家族成员共享类似的有源站点架构，但是AMBGL-LP在溶液中形成稳定的二聚体。通过Metagenomics鉴定新GH1成员可能会扩大我们对这些酶的分子机制和多样性的理解，除了使我们能够调查其工业应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号