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首页> 外文期刊>Biochemical Genetics >Cloning, heterologous expression, and characterization of the xylitol and L-arabitol dehydrogenase genes, Texdh and Telad, from the thermophilic fungus Talaromyces emersonii.
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Cloning, heterologous expression, and characterization of the xylitol and L-arabitol dehydrogenase genes, Texdh and Telad, from the thermophilic fungus Talaromyces emersonii.

机译：克隆，异源表达和木糖醇和le-阿拉伯醇脱氢酶基因，TEXDH和TELAD的表征，来自嗜热真菌塔罗米斯·艾默生。

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摘要

The genes encoding xylitol dehydrogenase (Texdh) and L: -arabitol dehydrogenase (Telad) are involved in the fungal pentose pathway and were isolated from the thermophilic fungus Talaromyces emersonii, expressed in Escherichia coli, and the products purified to homogeneity. TeXDH showed activity toward xylitol and D: -sorbitol. TeLAD was active with L: -arabitol, xylitol, and D: -sorbitol. Phylogenetic analysis showed TeLAD has evolved from D: -sorbitol dehydrogenase as a result of environmental adaptation. Substrate specificity studies indicate that TeXDH is likely to have evolved from the more broadly acting TeLAD. Texdh and Telad expression was inducible by the same carbon sources responsible for induction of genes involved in biomass degradation, suggesting for the first time a coordinated regulatory control mechanism for expression of genes encoding extracellular hydrolases and intracellular metabolic genes in the pentose utilization pathways of T. emersonii. These data also suggest that TeXDH and TeLAD may be valuable in the production of xylitol, L: -arabitol, and ethanol from renewable resources rich in pentose sugars.
机译：编码木糖醇脱氢酶（Texdh）和L：-Arabitol脱氢酶（Telad）的基因参与真菌戊糖途径，并从嗜热真菌塔罗米氏症中分离，在大肠杆菌中表达，并将产品纯化为均匀性。 TEXDH向木糖醇和D：-Sorbitol显示出活性。 Telad与L：-Arabitol，木糖醇和D：-Sorbitol有效。由于环境适应导致，Phylocyic分析显示Telad已从D：-Sorbitol脱氢酶中演变。底物特异性研究表明，Texdh可能从更广泛的Telad中发展。 Texdh和Telad表达是由相同的碳源诱导，该碳源负责诱导参与生物质降解的基因，这是第一次表达编码细胞外水解酶和细胞内代谢基因的协调调节控制机制在T.艾默生。这些数据还表明，TEXDH和Telad在富含戊糖中的可再生资源的木糖醇，L：氨基酚和乙醇中可能是有价值的。

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