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首页> 外文期刊>Biochemical Genetics >Mapping and expression analysis of chicken NDRG1 and NDRG3 genes.
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Mapping and expression analysis of chicken NDRG1 and NDRG3 genes.

机译：鸡NDRG1和NDRG3基因的映射和表达分析。

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摘要

N-myc downstream-regulated genes 1 and 3 (NDRG1 and NDRG3) are members of the alpha/beta hydrolase superfamily. Phylogenetic analysis of the family demonstrated that human NDRG1 and 3 belong to a subfamily. The mapping and gene expression patterns of these genes represent one step toward further investigation into their possible roles in the chicken (Gallus gallus). To map these genes in the chicken chromosome, a 6000 rads chicken-hamster radiation hybrid panel (ChickRH6) was used. Primers were designed according to the published human sequences for amplification of those two genes. We compared the corresponding human mRNA sequences with the predicted coding sequences of the chicken NDRG1 and 3 genes and found that the assembled contigs shared a high percentage of similarity with the human genes. PCR of samples from ChickRH6 revealed that the locations of NDRG1 and 3 are linked to the markers MYC (58 cRs away, LOD score 4.52) and SEQ0265 (10 cRs away, LOD score 17.81), respectively. This result adds two new markers to the chicken RH map, and it reinforces that the RH technique is indeed a powerful tool for mapping genes due to its rapidity, precision, convenience, and reproducibility. In addition, we detected the gene expression and distribution of chicken NDRG1 and 3 in seven tissues, including heart, liver, spleen, lung, muscle, brain, and thymus, by RT-PCR, and found that NDRG1 is relatively ubiquitously expressed in all the tested tissues and highly expressed in heart and liver, whereas NDRG3 is high in heart, muscle, and brain.
机译：N-MYC下游调节基因1和3（NDRG1和NDRG3）是α/β水解酶超家族的成员。家庭的系统发育分析证明人NDRG1和3属于亚家族。这些基因的映射和基因表达模式代表了进一步调查鸡肉（Gallus Gallus）的可能作用的一步。为了在鸡染色体中映射这些基因，使用6000弧度鸡肉 - 仓鼠辐射杂交面板（CHICKRH6）。根据公开的人序列设计了引物，用于扩增这两个基因。我们将相应的人mRNA序列与鸡NDRG1和3基因的预测编码序列进行了比较，发现组装的Contigs与人类基因共享高比例的相似性。来自ChickRH6的样品的PCR揭示了NDRG1和3的位置分别与标记物体（58 CRS掉，LOD评分4.52）和SEQ0265（10 CRS远离，LOD得分17.81）连接。该结果为鸡RH图增加了两个新标记，它强化了RH技术，确实是由于其快速，精确，方便和再现性而导致的映射基因的强大工具。此外，我们在七种组织中检测到鸡NDRG1和3的基因表达和分布，包括心脏，肝脏，脾，肺，肌肉，脑和胸腺，通过RT-PCR，发现NDRG1相对普遍地表达测试组织和心脏和肝脏高度表达，而NDRG3在心脏，肌肉和大脑中高。

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