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首页> 外文期刊>Biochemistry >Polyclonal Antibodies to LIM Proteins CRP2 and CRIP2 Reveal Their Subcellular Localizations in Olfactory Precursor Cells
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Polyclonal Antibodies to LIM Proteins CRP2 and CRIP2 Reveal Their Subcellular Localizations in Olfactory Precursor Cells

机译：对羟蛋白CRP2和CRIP2的多克隆抗体揭示了嗅前体细胞中的亚细胞局部

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摘要

In this study, we describe the presence of CRP2 (cysteine- and glycine-rich protein 2) and CRIP2 (cysteine-rich intestinal protein 2), which are members of group 2 LIM proteins, in rat olfactory precursor cells by reverse transcription polymerase chain reaction. We have developed polyclonal antibodies against CRP2 and CRIP2 individually. Specificity of the antibodies was demonstrated by Western blot analysis, using CRP2 and CRIP2 transfected cells. No cross-reactivity was observed between the antibodies. Furthermore, we used the antibodies to determine the expression and localization of CRP2 and CRIP2 in olfactory precursor cells by Western blot analysis and immunofluorescence staining. Our results demonstrated that in undifferentiated olfactory precursor cells CRP2 was distributed both in the nucleus and the cytoplasm, whereas CRIP2 was predominantly localized in the cytoplasm. While the olfactory precursor cells differentiated into end cells, only the expression of CRIP2 would be detected. The function of these LIM proteins in olfactory precursor cells warrants further study.
机译：在该研究中，我们描述了通过逆转录聚合酶链在大鼠嗅前体细胞中的CRP2（富含富含甘氨酸和富含甘氨酸蛋白2）和CRIP2（富含半胱氨酸和富含甘氨酸蛋白2）和CRIP2（富含半胱氨酸富肠蛋白2）的存在反应。我们已经单独开发了针对CRP2和CRIP2的多克隆抗体。通过蛋白质印迹分析来证明抗体的特异性，使用CRP2和CRIP2转染细胞来证明。在抗体之间没有观察到交叉反应性。此外，我们使用抗体通过Western印迹分析和免疫荧光染色来确定CRP2和CRIP2在嗅前体细胞中的表达和定位。我们的结果表明，在未分化的嗅到前体细胞中，CRP2在细胞核和细胞质中分布​​，而CRIP2主要归入细胞质。虽然将嗅觉前体细胞分化成末端细胞，但仅检测CRIP2的表达。这些Lim蛋白在嗅前体细胞中的功能需要进一步研究。

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  • 来源
    《Biochemistry》 |2009年第3期|共6页
  • 作者

    Xue Gao; Jing-Yu Sun; Zeng-Yu Cao;

  • 作者单位

    Department of Otorhinolaryngology Head and Neck Surgery Xijing Hospital Fourth Military Medical University Xi'an Shaanxi 710032 P. R. China;

    Department of Otorhinolaryngology Head and Neck Surgery Xijing Hospital Fourth Military Medical University Xi'an Shaanxi 710032 P. R. China;

    Department of Otorhinolaryngology Head and Neck Surgery Xijing Hospital Fourth Military Medical University Xi'an Shaanxi 710032 P. R. China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学;
  • 关键词

    CRP2; CRIP2; LIM protein; polyclonal antibody; olfactory precursor cells;

    机译：CRP2;CRIP2;LIM蛋白;多克隆抗体;嗅前体细胞;

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