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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Effects of the osmolyte TMAO (Trimethylamine-N-oxide) on aqueous hydrophobic contact-pair interactions
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Effects of the osmolyte TMAO (Trimethylamine-N-oxide) on aqueous hydrophobic contact-pair interactions

机译：Osmolyte TMAO（三甲胺-N-氧化物）对疏水性接触对相互作用的影响

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摘要

Osmolytes are small, soluble organic molecules produced by living organisms for maintaining cell volume. These molecules have also been shown to have significant effects on the stability of proteins. Perhaps one of the most studied osmolytes is Trimethylamine-N-oxide (TMAO). Thermodynamic studies of the effects of TMAO on proteins have shown that this molecule is a strong stabilizer of the protein folded state, thus being able to counteract the effects of protein denaturants such as urea and guanidine hydrochloride. Most studies of TMAO effects on bio-molecular stability have until now been focused on how the osmolyte reduces the solubility of polypeptide backbones, while the effects of TMAO on hydrophobic interactions are still not well understood. In fact, there are few experimental data measuring the effect of TMAO on hydrophobic interactions. This work studies phenyl and alkyl contact pairs as model hydrophobic contact pairs. The formation of these contact pairs is monitored using fluorescence, i.e., through the quenching of phenol fluorescence by carboxylate ions; and a methodology is developed to isolate hydrophobic contributions from other interactions. The data demonstrate that the addition of TMAO to the aqueous solvent destabilizes hydrophobic contact pairs formed between alkyl and phenyl moieties. In other words, TMAO acts as a "denaturant" for hydrophobic interactions.
机译：渗透物是由生物体产生的小型，可溶性有机分子用于维持细胞体积。这些分子也已被证明对蛋白质的稳定性具有显着影响。也许最多研究的渗透物之一是三甲胺-n-氧化物（TMAO）。 TMAO对蛋白质的影响的热力学研究表明，该分子是蛋白质折叠状态的强稳定剂，从而能够抵消蛋白质变性剂如尿素和盐酸胍蛋白酶的影响。大多数对生物分子稳定性的TMAO影响的研究迄今为止，渗透物如何降低多肽骨干骨干的溶解度，而TMAO对疏水相互作用的影响仍未得到很好的理解。事实上，少量测量TMAO对疏水相互作用的效果的实验数据。该工作研究苯基和烷基接触对作为模型疏水接触对。使用荧光，即通过羧酸盐离子淬火酚荧光来监测这些接触对的形成;和方法是开发出与其他相互作用的疏水贡献。数据表明，将TMAO添加到水性溶剂中稳定在烷基和苯基部分之间形成的疏水接触对。换句话说，TMAO充当疏水相互作用的“变性”。

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