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首页> 外文期刊>Breeding science >ISSR markers to assess genetic diversity of cultivated populations from artificial selection of Stevia rebaudiana (Bert.) Bertoni
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ISSR markers to assess genetic diversity of cultivated populations from artificial selection of Stevia rebaudiana (Bert.) Bertoni

机译：ISSR标记评估从史蒂维亚雷博尼亚（BERT.）Bertoni的人工选择培养种群的遗传多样性

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摘要

Artificial selection related with important agronomic characteristics of Stevia rebaudiana may cause genetic divergence and formation of genetically structured populations with genetic uniformity or diversity within cultivars. Current study employed inter simple sequence repeats of DNA (ISSR markers) to assess genetic diversity within and among a single cultivated population maintained through sexual propagation (SR1) and four cultivated populations generated by artificial selection and maintained by vegetative propagation (SR2-SR5). Highest polymorphism rate was reported in SR1 (89.24%), whilst the lowest rate of polymorphism occurred in SR2 (60.13%). ISSR markers revealed that selection of plants with traits of vegetative-propagated interest may lead towards the generation of genetically more uniform DNA-level populations, while plants maintained by sexual propagation have high genetic variability. High estimated genetic divergence level indicated that the five areas of stevia form genetically structured populations. SR2 and SR4 are constituted by plants more homogeneous at DNA level for the selected characteristics than plants of SR3 and SR5 populations. Predominant and homogeneous genotypes selected at SR2 and SR4 areas could be valuable for tracing strategies to obtain stcvia plants with the desirable agronomic characteristics through crosses between contrasting individuals in future breeding programs.
机译：与甜叶菊的重要农产性特征有关的人工选择可能导致遗传分歧和形成具有遗传均匀性或品种内的遗传均匀性或多样性。目前的研究采用DNA（ISSR标志物）的间简单序列重复，评估通过性繁殖（SR1）和由人工选择产生的四种培养种群的单一培养人群中的遗传多样性和由植物繁殖（SR2-SR5）维持。 SR1（89.24％）报告了最高的多态性率，而在SR2（60.13％）中发生的最低多态性速率最低。 ISSR标记显示，植物繁殖的兴趣具有特征的植物的选择可能导致转基因更均匀的DNA水平群体，而通过性繁殖维持的植物具有高遗传变异性。高估计的遗传分歧水平表明，甜叶菊的五个地区形成了遗传结构的群体。 SR2和SR4由比SR3和SR5种群的植物更均匀的DNA水平更均匀的植物构成。在SR2和SR4区域中选择的主要和均相基因型可能对追踪策略来获得STCVIA植物的策略，通过未来育种计划中的对比中的造影人之间的交叉来获得所需的农艺特征。

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