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首页> 外文期刊>Breeding science >Parsley ubiquitin promoter displays higher activity than the CaMV 35S promoter and the chrysanthemum actin 2 promoter for productive, constitutive, and durable expression of a transgene in Chrysanthemum morifolium
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Parsley ubiquitin promoter displays higher activity than the CaMV 35S promoter and the chrysanthemum actin 2 promoter for productive, constitutive, and durable expression of a transgene in Chrysanthemum morifolium

机译：欧芹泛素启动子显示比CAMV 35s启动子和菊花肌动蛋白2启动子更高的活性，用于在菊花Morifolium中的转基因的生产，本构体和耐用的转基因表达

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摘要

The chrysanthemum (Chrysanthemum morifolium) is one of the most popular ornamental plants in the world. Genetic transformation is a promising tool for improving traits, editing genomes, and studying plant physiology. Promoters are vital components for efficient transformation, determining the level, location, and timing of transgene expression. The cauliflower mosaic virus (CaMV) 35S promoter is most frequently used in dicotyledonous plants but is less efficient in chrysanthemums than in tobacco or torenia plants. Previously, we used the parsley ubiquitin (PcUbi) promoter in chrysanthemums for the first time and analyzed its activity in transgenic calli. To expand the variety of constitutive promoters in chrysanthemums, we cloned the upstream region of the actin 2 (CmACT2) gene and compared its promoter activity with the 35S and PcUbi promoters in several organs, as well as its durability for long-term cultivation. The CmACT2 promoter has higher activity than the 35S promoter in calli but is less durable. The PcUbi promoter has the highest activity not only in calli but also in leaves, ray florets, and disk florets, and retains its activity after long-term cultivation. In conclusion, we have provided useful information and an additional type of promoter available for transgene expression in chrysanthemums.
机译：菊花（Chrysanthemum Morifolium）是世界上最受欢迎的观赏植物之一。遗传转化是提高特质，编辑基因组和学习植物生理学的有前途的工具。启动子是有效转化的重要组成部分，确定转基因表达的水平，位置和时序。花椰菜马赛克病毒（CAMV）35s启动子最常用于双子叶植物，但在菊花中的效率低于烟草或托牙植物。以前，我们首次使用荷苗中的欧芹泛素（PCubi）启动子并分析其在转基因愈伤组织中的活性。为了扩大菊花中的各种组成型启动子，我们克隆了肌动蛋白2（CMact2）基因的上游区域，并将其在几个器官中与35s和pcubi启动子的启动子活性相比，以及其长期培养的耐久性。 CMact2启动子的活性高于Calli的35s启动子，但不太耐用。 PCUBI启动子不仅在Calli中的最高活动，而且在叶子，雷弗罗特和磁盘小花中，并在长期培养后保留其活动。总之，我们提供了有用的信息和可用于菊花中的转基因表达的另一种类型的启动子。

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