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首页> 外文期刊>Biochemistry and molecular biology education >Approaching the thermodynamic view of protein folding through the reproduction of Anfinsen's experiment by undergraduate physical biochemistry students
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Approaching the thermodynamic view of protein folding through the reproduction of Anfinsen's experiment by undergraduate physical biochemistry students

机译：通过本科物理生物化学学生繁殖Anfinsen实验的繁殖蛋白质折叠的热力学视图

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In 1972 Christian B. Anfinsen received the Nobel Prize in Chemistry for ...his work on ribonuclease, especially concerning the connection between the amino acid sequence and the biologically active conformation. The understanding of this principle is crucial for physical biochemistry students, since protein folding studies, bio-computing sciences and protein design approaches are founded on such a well-demonstrated connection. Herein, we describe a detailed and easy-to-follow experiment to reproduce the most relevant assays carried out at Anfinsen's laboratory in the 60s. This experiment provides students with a platform to interpret by themselves the structural and kinetic experiments conceived to understand the protein folding problem. In addition, this three-day experiment brings students a nice opportunity for protein manipulation as well as for the setting up of spectroscopic and chromatographic techniques. (c) 2018 by The International Union of Biochemistry and Molecular Biology, 46(3):262-269, 2018.
机译：1972年，基督教B. Anfinsen在化学中获得了诺贝尔奖的...他对核糖核酸酶的作品，特别是关于氨基酸序列与生物学主动构象之间的连接。对这一原则的理解对于物理生化学生来说至关重要，因为蛋白质折叠研究，生物计算科学和蛋白质设计方法都建立在如此熟悉的连接上。在此，我们描述了一种详细且易于遵循的实验，以在60年代中繁殖在Anfinsen的实验室中进行的最相关的测定。该实验为学生提供了一个平台来解释本身的结构和动力学实验，以了解蛋白质折叠问题。此外，这三天的实验还为学生带来了蛋白质操作的良好机会，以及设置光谱和色谱技术。 （c）2018由国际生物化学联盟和分子生物学，46（3）：262-269,2018。

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