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首页> 外文期刊>Biogerontology >Detecting senescent fate in mesenchymal stem cells: a combined cytofluorimetric and ultrastructural approach
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Detecting senescent fate in mesenchymal stem cells: a combined cytofluorimetric and ultrastructural approach

机译：检测间充质干细胞中的衰老命运：组合细胞荧光和超微结构方法

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摘要

Senescence can impair the therapeutic potential of stem cells. In this study, senescence-associated morphofunctional changes in periosteum-derived progenitor cells (PDPCs) from old and young individuals were investigated by combining cytofluorimetry, immunohistochemistry, and transmission electron microscopy. Cell cycle analysis demonstrated a large number of G0/G1 phase cells in PDPCs from old subjects and a progressive accumulation of G0/G1 cells during passaging in cultures from young subjects. Cytofluorimetry documented significant changes in light scattering parameters and closely correlated with the ultrastructural features, especially changes in mitochondrial shape and autophagy, which are consistent with the mitochondrial-lysosomal axis theory of ageing. The combined morphological, biofunctional, and ultrastructural approach enhanced the flow cytometric study of PDPC ageing. We speculate that impaired autophagy, documented in replicative senescent and old PDPCs, reflect a switch from quiescence to senescence. Its demonstration in a tissue with limited turnover—like the cambium layer of the periosteum, where reversible quiescence is the normal stem cell state throughout life—adds a new piece to the regenerative medicine jigsaw in an ageing society.
机译：衰老会损害干细胞的治疗潜力。在该研究中，通过组合细胞杂氟杂法，免疫组织化学和透射电子显微镜来研究来自旧和年轻个体的颅脑衍生的祖细胞（PDPC）的衰老相关的形态官能变化。细胞循环分析显示来自旧对象的PDPC中的大量G0 / G1相细胞和在从年轻受试者的文化中传代期间G0 / G1细胞的渐进积累。细胞流氟化物记录了光散射参数的显着变化，并与超微结构特征密切相关，特别是线粒体形状和自噬的变化，这与老化的线粒体 - 溶酶体轴理论一致。组合的形态学，生物功能和超微结构方法增强了PDPC老化的流式细胞术研究。我们推测，在复制衰老和旧的PDPC中记录的自噬受损，反映了从静态到衰老的切换。它在具有有限的周转型骨膜夹层的组织中的演示，其中可逆的静态是整个生命中的正常干细胞状态 - 在老龄化社会中增加了一件新的药物拼图。

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