...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell biology international. >Urothelial differentiation of human amniotic fluid stem cells by urothelium specific conditioned medium.
【24h】

Urothelial differentiation of human amniotic fluid stem cells by urothelium specific conditioned medium.

机译：尿液细胞尿液中尿液液体干细胞尿路上分化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Human amniotic fluid stem cells (HAFSCs) have a high proliferative capacity and a good differentiation potential, and may thus be suitable for regenerative medicine. To date, urothelial differentiation mechanisms of HAFSCs are poorly understood. We have investigated the urothelial differentiation potential of HAFSCs so that they can be therapeutically applied to cure defective diseases of bladder. To induce the stem cell differentiation, HAFSCs were cultured in a bladder cancer-derived conditioned medium. After 2 weeks of culture, HAFSCs began to express the urothelial lineage-specific markers (UPII, CK8 and FGF10). Meanwhile, pluripotency markers (Oct-4, Sox-2 and Nanog) were downregulated at both RNA and protein levels in the differentiated HAFSCs. Immunocytochemistry data revealed that differentiated HAFSCs expressed urothelial markers of UPII and CK8. We have screened the receptor tyrosine kinase arrays with the differentiated HAFSCs. The screening showed that MuSK, Tie-1 and EphA4 receptor tyrosine kinases were upregulated, whereas EphA7 and FGF R1 kinases were downregulated in HAFSCs. The data suggest that HAFSCs can be an important urothelium cell source, which can be used for urinary tract engineering.
机译：人羊水干细胞（HAFSCs）具有高增殖能力和良好的分化势，因此可以适用于再生药。迄今为止，HAFSCS的尿路上分化机制明显不知所措。我们研究了HAFSCS的尿路上分化潜力，以便可以治疗膀胱缺陷的治疗方法。为了诱导干细胞分化，HAFSCs在膀胱癌衍生的条件培养基中培养。培养2周后，HAFSCS开始表达尿路上的谱系特异性标记（UPII，CK8和FGF10）。同时，在分化的HAFSCs中的RNA和蛋白质水平下，多能性标记（OCT-4，SOX-2和NANOG）下调。免疫细胞化学数据显示，分化的HAFSCs表达UPII和CK8的尿路上皮标记。我们用分化的HAFSC筛选受体酪氨酸激酶阵列。筛选显示麝香，Tie-1和Epha4受体酪氨酸激酶被上调，而Epha7和FGF R1激酶在HAFSCs中下调。数据表明HAFSCS可以是重要的尿路升性电池源，其可用于泌尿道工程。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号