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首页> 外文期刊>Cell and Tissue Research >Identification of M2 macrophages in anterior pituitary glands of normal rats and rats with estrogen-induced prolactinoma
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Identification of M2 macrophages in anterior pituitary glands of normal rats and rats with estrogen-induced prolactinoma

机译：雌激素诱导术术术大鼠垂体大鼠垂体腺体中M2巨噬细胞的鉴定

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Macrophages are present throughout the anterior pituitary gland. However, the features and function of macrophages in the gland are poorly understood. Recent studies have indicated that there are two main macrophage classes: M1 (classically activated) and M2 (alternatively activated). In this study, we examine whether both M1 and M2 macrophages are present in the anterior pituitary gland of rats. Our findings indicate that macrophages that are positive for CD68 (a pan-macrophage marker) were localized near capillaries in rat anterior pituitary gland. These macrophages were positive for iNOS or mannose receptor (MR), which are markers of M1 and M2 macrophages, respectively. To determine the morphological characteristics of M2 macrophages under pathological conditions, diethylstilbestrol (DES)-treated rats were used as an animal model of prolactinoma. After 2 weeks of DES treatment, a number of MR-immunopositive cells were present in the gland. Immunoelectron microscopy revealed that MR-immunopositive M2 macrophages had many small vesicles and moderately large vacuoles in cytoplasm. Phagosomes were sometimes present in cytoplasm. Interestingly, M2 macrophages in prolactinoma tissues did not usually exhibit distinct changes or differences during the normal, hyperplasia and adenoma stages. This study is the first to confirm that both M1 and M2 macrophages are present in the anterior pituitary gland of rats. Moreover, the number of M2 macrophages was greatly increased in rats with DES-induced prolactinoma. Future studies should attempt to characterize the functional role of M2 macrophages in the gland.
机译：巨噬细胞在整个垂体腺体中存在。然而，腺体中巨噬细胞的特征和功能被理解得很差。最近的研究表明存在两种主要的巨噬细胞等级：M1（经典激活）和M2（可选地激活）。在这项研究中，我们检查大鼠前脑前腺体中是否存在M1和M2巨噬细胞。我们的研究结果表明，CD68阳性的巨噬细胞（泛巨噬细胞标志物）局部局部大鼠前脑前床上的毛细血管附近。这些巨噬细胞对于Inos或甘露糖受体（MR）分别是M1和M2巨噬细胞的标志物。为了确定病理条件下M2巨噬细胞的形态学特征，二乙基胱在DES治疗后2周后，腺体中存在许多MR-IMMUN倍数细胞。免疫电解显微镜显示MR-IMMUN阳性M2巨噬细胞在细胞质中具有许多小囊泡和中等大型液泡。吞噬体有时存在于细胞质中。有趣的是，M2腭瘤组织中的M2巨噬细胞通常不会在正常，增生和腺瘤阶段表现出不同的变化或差异。本研究是第一个证实M1和M2巨噬细胞的大鼠垂体腺体中的巨噬细胞都存在。此外，在DES诱导的术语术语大鼠中，M2巨噬细胞的数量大大增加。未来的研究应该试图表征M2巨噬细胞在腺体中的功能作用。

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