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首页> 外文期刊>Cell and Tissue Research >PPAR gamma activation regulates lipid droplet formation and lactate production in rat Sertoli cells
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PPAR gamma activation regulates lipid droplet formation and lactate production in rat Sertoli cells

机译：PPARγ活化调节大鼠血液液滴形成和乳酸乳液产生

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摘要

Sertoli cells provide the structural and nutritional support for germ cell development; they actively metabolize glucose and convert it to lactate, which is an important source of energy for germ cells. Furthermore, Sertoli cells can oxidize fatty acids, a metabolic process that is assumed to fulfill their own energy requirements. Fatty acids are stored as triacylglycerides within lipid droplets. The regulation of fatty acid storage in conjunction with the regulation of lactate production may thus be relevant to seminiferous tubule physiology. Our aim is to evaluate a possible means of regulation by the PPAR gamma activation of lipid droplet formation and lactate production. Sertoli cell cultures obtained from 20-day-old rats were incubated with Rosiglitazone (10 mu M), a PPAR gamma activator, for various periods of time (6, 12, 24 and 48 h). Increased triacylglycerides levels and lipid droplet content were observed, accompanied by a rise in the expression of genes for proteins involved in fatty acid storage, such as the fatty acid transporter Cd36, glycerol-3-phosphate-acyltransferases 1 and 3, diacylglycerol acyltransferase 1 and perilipins 1, 2 and 3, all proteins that participate in lipid droplet formation and stabilization. However, PPAR gamma activation increased lactate production, accompanied by an augmentation in glucose uptake and Glut2 expression. These results taken together suggest that PPAR gamma activation in Sertoli cells participates in the regulation of lipid storage and lactate production thereby ensuring simultaneously the energetic metabolism for the Sertoli and germ cells.
机译：Sertoli细胞为生殖细胞发育提供了结构和营养支持;他们积极地代谢葡萄糖并将其转化为乳酸，这是生殖细胞的重要能量来源。此外，Sertoli细胞可以氧化脂肪酸，这是一种代谢过程，假设满足自己的能量要求。脂肪酸作为三酰基甘油酯储存在脂质液滴中。因此，脂肪酸储存与乳酸乳酸盐产生的调节可能与嗜血水池生理学相关。我们的目标是通过PPARγ激活脂液滴形成和乳酸生产来评估可能的调节手段。从20天龄大鼠获得的血清细胞培养物与罗格列酮（10μm），PPARγ激活剂，各个时段（6,12,24和48小时）温育。观察到增加的三酰基甘油水平和脂质液滴含量，伴随着参与脂肪酸储存的蛋白质基因的基因的表达，例如脂肪酸转运蛋白CD36，甘油-3-磷酸酰基 - 转移酶1和3，二酰基甘油酰基转移酶1和Perilipins 1,2和3，所有参与脂液滴形成和稳定化的蛋白质。然而，PPARγ活化增加乳酸盐产生，伴有葡萄糖摄取和Glut2表达的增强。这些结果表明，Sertoli细胞中的PPARγ活化参与脂质储存和乳酸生成的调节，从而同时确保了Sertoli和生殖细胞的能量代谢。

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  • 来源
    《Cell and Tissue Research》 |2017年第3期|共14页
  • 作者

    Gorga A.; Rindone G. M.; Regueira M.; Pellizzari E. H.; Camberos M. C.; Cigorraga S. B.; Riera M. F.; Galardo M. N.; Meroni S. B.;

  • 作者单位

    Hosp Ninos Dr Ricardo Gutierrez Ctr Invest Endocrinol Dr Cesar Bergada CONICET FEI Div Endocrinol;

    Hosp Ninos Dr Ricardo Gutierrez Ctr Invest Endocrinol Dr Cesar Bergada CONICET FEI Div Endocrinol;

    Hosp Ninos Dr Ricardo Gutierrez Ctr Invest Endocrinol Dr Cesar Bergada CONICET FEI Div Endocrinol;

    Hosp Ninos Dr Ricardo Gutierrez Ctr Invest Endocrinol Dr Cesar Bergada CONICET FEI Div Endocrinol;

    Hosp Ninos Dr Ricardo Gutierrez Ctr Invest Endocrinol Dr Cesar Bergada CONICET FEI Div Endocrinol;

    Hosp Ninos Dr Ricardo Gutierrez Ctr Invest Endocrinol Dr Cesar Bergada CONICET FEI Div Endocrinol;

    Hosp Ninos Dr Ricardo Gutierrez Ctr Invest Endocrinol Dr Cesar Bergada CONICET FEI Div Endocrinol;

    Hosp Ninos Dr Ricardo Gutierrez Ctr Invest Endocrinol Dr Cesar Bergada CONICET FEI Div Endocrinol;

    Hosp Ninos Dr Ricardo Gutierrez Ctr Invest Endocrinol Dr Cesar Bergada CONICET FEI Div Endocrinol;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    PPAR gamma; Sertoli cells; Lipid droplets; Lactate; Perilipins;

    机译：PPARγ;血清细胞;脂滴;乳酸;泛脂;

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