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首页> 外文期刊>Chemical and Pharmaceutical Bulletin >Simultaneous Quantification of Seven Constituents from Zaoren Anshen Prescription and Four Endogenic Components in Rat Plasma by UHPLC-TSQ-MS/MS and the Application of the Correlation Study
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Simultaneous Quantification of Seven Constituents from Zaoren Anshen Prescription and Four Endogenic Components in Rat Plasma by UHPLC-TSQ-MS/MS and the Application of the Correlation Study

机译：通过UHPLC-TSQ-MS / MS同时定量来自Zaoren Anshen处方和大鼠血浆中的四个内部根成分的组分及其相关研究的应用

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摘要

A highly rapid and sensitive ultra-performance liquid chromatography-tandem triple quadrupole mass spectrometry (UHPLC-TSQ-MS/MS) method was developed for the simultaneous determination of 7 constituents from Zaoren Anshen prescription (ZAP) and 4 endogenic components in rat plasma. The proteins in the plasma samples were removed using acetonitrile. The separation of the 11 components was performed on an Alltima C18 column (150 x 4.6 mm, 5 mu m) with acetonitrile and 0.01% formic acid water as the mobile phase. Quantification of the 11 components was performed by multiple reaction monitoring (MRM), and the electrospray ion source polarity was switched between positive and negative modes. The method exhibited good linearity for the 11 components (R-2>0.9942). The lower quantitative limit for the 11 components was in the range from 0.90-9.95 ng/mL. The precision was evaluated by intraday and interday assays, with all relative standard deviation (RSD)% values within 14.92%. The relative error of the accuracy ranged from -9.90 to 14.93%. The recovery ranged from 73.94 to 101.06%, and the matrix effects of the 7 components ranged from 80.06 to 105.70%. The developed method was successfully applied for correlation analysis for the simultaneous quantification of the 7 constituents from ZAP and 4 endogenic components in rat plasma after ZAP treatment.
机译：开发出高度快速敏感的超高效液相色谱 - 串联三重四极杆质谱（UHPLC-TSQ-MS / MS）方法，用于同时测定来自Zaoren Anshen处方（ZAP）和大鼠等离子体中的4个内源组分的组分。使用乙腈除去等离子体样品中的蛋白质。将11个组分的分离在Alltima C18柱（150×4.6mm，5μm）上，用乙腈和0.01％甲酸水作为流动相。通过多次反应监测（MRM）进行11个组分的定量，并且电喷雾离子源极性在正极和负模式之间切换。该方法表现出11分量的良好线性度（R-2> 0.9942）。 11个组分的较低定量极限在0.90-9.95ng / ml的范围内。通过盘中和白天测定评估精度，所有相对标准偏差（RSD）％值均为14.92％。精度的相对误差范围为-9.90至14.93％。恢复的范围从73.94到101.06％，7个组分的基质效应范围为80.06至105.70％。已成功应用于ZAP处理后大鼠血浆中7种成分的同时定量7种成分的相关分析的相关性分析。

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