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首页> 外文期刊>Acta biomaterialia >Hydrogels derived from demineralized and decellularized bone extracellular matrix.
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Hydrogels derived from demineralized and decellularized bone extracellular matrix.

机译：水凝胶来自脱矿质和脱细胞骨细胞外基质的水凝胶。

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The extracellular matrix (ECM) of mammalian tissues has been isolated, decellularized and utilized as a scaffold to facilitate the repair and reconstruction of numerous tissues. Recent studies have suggested that superior function and complex tissue formation occurred when ECM scaffolds were derived from site-specific homologous tissues compared with heterologous tissues. The objectives of the present study were to apply a stringent decellularization process to demineralized bone matrix (DBM), prepared from bovine bone, and to characterize the structure and composition of the resulting ECM materials and DBM itself. Additionally, we sought to produce a soluble form of DBM and ECM which could be induced to form a hydrogel. Current clinical delivery of DBM particles for treatment of bone defects requires incorporation of the particles within a carrier liquid. Differences in osteogenic activity, inflammation and nephrotoxicity have been reported with various carrier liquids. The use of hydrogel forms of DBM or ECM may reduce the need for carrier liquids. DBM and ECM hydrogels exhibited sigmoidal gelation kinetics consistent with a nucleation and growth mechanism, with ECM hydrogels characterized by lower storage moduli than the DBM hydrogels. Enhanced proliferation of mouse primary calvarial cells was achieved on ECM hydrogels, compared with collagen type I and DBM hydrogels. These results show that DBM and ECM hydrogels have distinct structural, mechanical and biological properties and have the potential for clinical delivery without the need for carrier liquids.
机译：哺乳动物组织的细胞外基质（ECM）已被隔离，脱细胞，用作支架，以促进许多组织的修复和重建。最近的研究表明，当与异源组织相比，当ECM支架衍生自特异性同源组织时，发生了优异的功能和复杂组织形成。本研究的目的是将严格的脱细胞化方法应用于从牛骨制备的脱矿质骨基质（DBM），并表征所得ECM材料和DBM本身的结构和组成。另外，我们寻求产生一种可溶形式的DBM和ECM，其可以被诱导形成水凝胶。目前用于治疗骨缺陷的DBM颗粒的临床递送需要将颗粒掺入载体液体内。已经报道了各种载体液体的成骨活性，炎症和肾毒性的差异。使用水凝胶形式的DBM或ECM可以减少对载体液体的需求。 DBM和ECM水凝胶表现出与成核和生长机制一致的六样凝胶化动力学，具有比DBM水凝胶更低的储存模量的ECM水凝胶。与胶原蛋白I型和DBM水凝胶相比，在ECM水凝胶上达到了对小鼠初级颅骨细胞的增强的增殖。这些结果表明，DBM和ECM水凝胶具有不同的结构，机械和生物学性质，具有临床递送的可能性，而无需载体液体。

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