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首页> 外文期刊>Acta biomaterialia >Nanostructural control of the release of macromolecules from silica sol-gels.
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Nanostructural control of the release of macromolecules from silica sol-gels.

机译：二氧化硅溶胶 - 凝胶释放大分子释放的纳米结构控制。

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The therapeutic use of biological molecules such as growth factors and monoclonal antibodies is challenging in view of their limited half-life in vivo. This has elicited the interest in delivery materials that can protect these molecules until released over extended periods of time. Although previous studies have shown controlled release of biologically functional BMP-2 and TGF-β from silica sol-gels, more versatile release conditions are desirable. This study focuses on the relationship between room temperature processed silica sol-gel synthesis conditions and the nanopore size and size distribution of the sol-gels. Furthermore, the effect on release of large molecules with a size up to 70kDa is determined. Dextran, a hydrophilic polysaccharide, was selected as a large model molecule at molecular sizes of 10, 40 and 70kDa, as it enabled us to determine a size effect uniquely without possible confounding chemical effects arising from the various molecules used. Previously, acid catalysis was performed at a pH value of 1.8 below the isoelectric point of silica. Herein the silica synthesis was pursued using acid catalysis at either pH 1.8 or 3.05 first, followed by catalysis at higher values by adding base. This results in a mesoporous structure with an abundance of pores around 3.5nm. The data show that all molecular sizes can be released in a controlled manner. The data also reveal a unique in vivo approach to enable release of large biological molecules: the use more labile sol-gel structures by acid catalyzing above the pH value of the isoelectric point of silica; upon immersion in a physiological fluid the pores expand to reach an average size of 3.5nm, thereby facilitating molecular out-diffusion.
机译：鉴于其体内有限的半衰期，生长因子和单克隆抗体如生长因子和单克隆抗体的治疗用途是具有挑战性的。这引发了对递送材料的兴趣，可以保护这些分子直至在延长的时间内释放。尽管先前的研究表明，来自二氧化硅溶胶 - 凝胶的生物功能性BMP-2和TGF-β的控制释放，但是希望更通用的释放条件。本研究专注于室温处理二氧化硅溶胶 - 凝胶合成条件的关系以及溶胶凝胶的纳米孔尺寸和尺寸分布。此外，确定对尺寸高达70kDA的大分子释放的影响。葡聚糖，一种亲水多糖，作为10,40和70kDA的分子尺寸的大型模型分子选择，因为它使我们能够独特地确定尺寸效应而不可能从所用的各种分子产生的混淆化学效果。以前，在二氧化硅的等电点低于1.8的pH值的pH值下进行酸催化。本文首先使用pH 1.8或3.05的酸催化来追踪二氧化硅合成，然后通过加入碱在较高值下催化。这导致介孔结构，孔隙约为3.5nm。数据表明，所有分子尺寸都可以以受控的方式释放。数据还揭示了一种独特的体内方法，以实现大型生物分子的释放：通过酸催化使用更多不稳定的溶胶 - 凝胶结构，高于二氧化硅等电点的pH值;在浸入生理流体时，孔的膨胀率达到3.5nm的平均尺寸，从而促进分子出扩散。

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