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首页> 外文期刊>Acta biomaterialia >Monocyte/macrophage cytokine activity regulates vascular smooth muscle cell function within a degradable polyurethane scaffold
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Monocyte/macrophage cytokine activity regulates vascular smooth muscle cell function within a degradable polyurethane scaffold

机译：单核细胞/巨噬细胞细胞因子活性调节可降解聚氨酯支架内的血管平滑肌细胞功能

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摘要

Tissue engineering strategies rely on the ability to promote cell proliferation and migration into porous biomaterial constructs, as well as to support specific phenotypic states of the cells in vitro. The present study investigated the use of released factors from monocytes and their derived macrophages (MDM) and the mechanism by which they regulate vascular smooth muscle cell (VSMC) response in a VSMC-monocyte co-culture system within a porous degradable polyurethane (D-PHI) scaffold. VSMCs cultured in monocyte/MDM-conditioned medium (MCM), generated from the culture of monocytes/MDM on D-PHI scaffolds for up to 28 days, similarly affected VSMC contractile marker expression, growth and three-dimensional migration when compared to direct VSMC-monocyte co-culture. Monocyte chemotactic protein-1 (MCP-1) and interleukin-6 (IL-6) were identified as two cytokines present in MCM, at concentrations that have previously been shown to influence VSMC phenotype. VSMCs cultured alone on D-PHI scaffolds and exposed to MCP-1 (5 ng ml-1) or IL-6 (1 ng ml-1) for 7 days experienced a suppression in contractile marker expression (with MCP-1 or IL-6) and increased growth (with MCP-1) compared to no cytokine medium supplementation. These effects were also observed in VSMC-monocyte co-culture on D-PHI. Neutralization of IL-6, but not MCP-1, was subsequently shown to decrease VSMC growth and enhance calponin expression for VSMC-monocyte co-cultures on D-PHI scaffolds for 7 days, implying that IL-6 mediates VSMC response in monocyte-VSMC co-cultures. This study highlights the use of monocytes and their derived macrophages in conjunction with immunomodulatory biomaterials, such as D-PHI, as agents for regulating VSMC response, and demonstrates the importance of monocyte/MDM-released factors, such as IL-6 in particular, in this process.
机译：组织工程策略依赖于促进细胞增殖和迁移到多孔生物材料构建体中的能力，以及支持体外细胞的特定表型状态。本研究研究了使用单核细胞和衍生巨噬细胞（MDM）的释放因子和它们调节在多孔可降解聚氨酯内VSMC-单核细胞共培养系统中的血管平滑肌细胞（VSMC）反应的机制（D- Phi）脚手架。在单核细胞/ MDM条件培养基（MCM）中培养的VSMCs，从单核细胞/ MDM培养到D-PHI支架上长达28天，类似地影响VSMC收缩标记表达，生长和三维迁移与直接VSMC相比-MONOCYTE共同培养。单核细胞趋化蛋白-1（MCP-1）和白细胞介素-6（IL-6）被鉴定为在MCM中存在的两种细胞因子，以先前显示影响VSMC表型的浓度。仅在D-PHI支架上单独培养的VSMCs并暴露于MCP-1（5ng ML-1）或IL-6（1ng ML-1）7天内的收缩标记表达中的抑制（用MCP-1或IL- 6）与无细胞因子培养基补充相比，增加生长（用MCP-1）增加。在D-PHI上的VSMC-单核细胞共培养中也观察到这些效果。随后显示IL-6的中和，但不是MCP-1，以降低VSMC生长，增强VSMC-单核细胞共培养物对D-PHI支架7天的钙诺宁表达，暗示IL-6在单核细胞中介导VSMC反应 - VSMC共同文化。该研究突出了单核细胞和它们的衍生巨噬细胞与免疫调节生物材料（例如D-PH））作为用于调节VSMC反应的药剂，并表明单核细胞/ MDM释放因子的重要性，特别是IL-6，在这个过程中。

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  • 来源
    《Acta biomaterialia》 |2014年第3期|共10页
  • 作者

    BattistonK.G.; OuyangB.; LabowR.S.; SimmonsC.A.; SanterreJ.P.;

  • 作者单位

    Institute of Biomaterials and Biomedical Engineering University of Toronto Toronto ON M5S 3G9;

    Institute of Biomaterials and Biomedical Engineering University of Toronto Toronto ON M5S 3G9;

    Division of Cardiac Surgery University of Ottawa Heart Institute Ottawa ON K1Y 4W7 Canada;

    Institute of Biomaterials and Biomedical Engineering University of Toronto Toronto ON M5S 3G9;

    Institute of Biomaterials and Biomedical Engineering University of Toronto Toronto ON M5S 3G9;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;
  • 关键词

    Co-culture; Cytokines; Monocyte; Polyurethane; Smooth muscle cell;

    机译：共同培养;细胞因子;单核细胞;聚氨酯;平滑肌细胞;

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