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首页> 外文期刊>Acta biomaterialia >Porous membrane with reverse gradients of PDGF-BB and BMP-2 for tendon-to-bone repair: In vitro evaluation on adipose-derived stem cell differentiation
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Porous membrane with reverse gradients of PDGF-BB and BMP-2 for tendon-to-bone repair: In vitro evaluation on adipose-derived stem cell differentiation

机译：具有PDGF-BB的反向梯度的多孔膜和BMP-2用于肌腱 - 骨修复：对脂肪衍生的干细胞分化的体外评估

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摘要

Polycaprolactone (PCL)/Pluronic F127 membrane with reverse gradients of dual platelet-derived growth factor-β (PDGF-BB) and bone morphogenetic protein 2 (BMP-2) concentrations was fabricated using a diffusion method to investigate the effect of reverse gradients of dual growth factor concentrations on adipose-derived stem cell (ASC) differentiations, such as tenogenesis and osteogenesis. The PDGF-BB and BMP-2 were continuously released from the membrane for up to 35 days, with reversely increasing/decreasing growth factors along the membrane length. Human ASCs were seeded on the membrane with reverse PDGF-BB and BMP-2 gradients. The cells were confluent after 1 week of culture, regardless of growth factor types or concentrations on the membrane. Gene expression (real-time polymerase chain reaction), Western blot and immunohistological analyses after 1 and 2 weeks of ASC culture showed that the membrane sections with higher PDGF-BB and lower BMP-2 concentrations provided a better environment for ASC tenogenesis, while the membrane sections with higher BMP-2 and lower PDGF-BB concentrations were better for promoting osteogenesis. The results suggest that the membrane with reverse gradients of PDGF-BB and BMP-2 may be promising for tendon-to-bone repair, as most essential biological processes are mediated by gradients of biological molecules in the body.
机译：使用扩散方法制造具有双血小板衍生的生长因子-β（PDGF-BB）和骨形态发生蛋白2（BMP-2）浓度的反向梯度的聚己内酯（PCL）/ PLURONIC F127膜。研究反向梯度的效果双生长因子浓度对脂肪衍生的干细胞（ASC）差异，例如胎生和骨质发生。将PDGF-BB和BMP-2从膜连续释放到35天，随着膜长度的反向增加/降低生长因子。用反向PDGF-BB和BMP-2梯度将人ASC播种在膜上。无论膜上的生长因子类型或浓度如何，细胞在培养1周后融合。在ASC培养的1和2周后，基因表达（实时聚合酶链反应），蛋白质印迹和免疫组织学分析表明，具有较高PDGF-BB和较低的BMP-2浓度的膜部分为ASC胎素提供了更好的环境，而且具有较高BMP-2和降低PDGF-BB浓度的膜部分优选促进骨质发生。结果表明，具有PDGF-BB和BMP-2的反向梯度的膜可能对肌腱 - 骨骼修复有望，因为大多数必要的生物过程由体内生物分子的梯度介导。

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  • 来源
    《Acta biomaterialia》 |2014年第3期|共8页
  • 作者

    MinH.K.; OhS.H.; LeeJ.M.; ImG.I.; LeeJ.H.;

  • 作者单位

    Department of Advanced Materials Hannam University Daejeon 305-811 South Korea;

    Department of Nanobiomedical Science and WCU Center Dankook University Cheonan 330-714 South;

    Department of Orthopedics Dongguk University International Hospital Goyang 411-373 South Korea;

    Department of Orthopedics Dongguk University International Hospital Goyang 411-373 South Korea;

    Department of Advanced Materials Hannam University Daejeon 305-811 South Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;
  • 关键词

    Adipose stem cell; Differentiation; Growth factor gradient; Membrane; Tendon-to-bone;

    机译：脂肪干细胞;分化;生长因子梯度;膜;肌腱骨;

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