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首页> 外文期刊>Acta biomaterialia >Platelet-derived growth-factor-releasing aligned collagen-nanoparticle fibers promote the proliferation and tenogenic differentiation of adipose-derived stem cells
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Platelet-derived growth-factor-releasing aligned collagen-nanoparticle fibers promote the proliferation and tenogenic differentiation of adipose-derived stem cells

机译：血小板衍生的生长因子释放对齐的胶原纳米颗粒纤维促进脂肪衍生的干细胞的增殖和遗传分化

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摘要

In order to enhance the healing potential of an injured tendon, we have prepared a novel biomimetic aligned collagen-nanoparticle (NP) composite fiber using an electrochemical process. The aligned collagen-NP composite fiber is designed to affect the cellular activity of adipose-derived stem cells (ADSCs) through two different ways: (i) topographic cues from the alignment of collagen fibril and (ii) controlled release of platelet-derived growth factors (PDGFs) from the NPs. PDGF released from collagen-NP fibers significantly enhanced the proliferation of ADSCs when tested for up to 7 days. Moreover, compared to random collagen fibers with PDGFs, aligned collagen-NP fibers significantly promoted the desirable tenogenic differentiation of ADSCs, as evidenced by an increased level of tendon markers such as tenomodulin and scleraxis. On the other hand, no undesirable osteogenic differentiation, as measured by the unchanged level of alkaline phosphatase and osteocalcin, was observed. Together, these results indicate that the aligned collagen-NP composite fiber induced the tenogenic differentiation of ADSCs through both a topographic cue (aligned collagen fibril) and a chemical cue (PDGF released from NPs). Thus, our novel aligned collagen-NP composite fiber has a significant potential to be used for tendon tissue engineering and regeneration.
机译：为了增强受伤肌腱的愈合潜力，我们使用电化学方法制备了一种新的仿生对准的胶原型胶囊（NP）复合纤维。对齐的胶原蛋白-NP复合纤维被设计为通过两种不同的方式影响脂肪衍生的干细胞（ADSC）的细胞活性：（i）来自胶原纤维纤维纤维和（ii）的血小板衍生生长的控制释放的地形提示来自NPS的因素（PDGFS）。从胶原蛋白-NP纤维释放的PDGF在测试最多7天内显着增强了ADSC的增殖。此外，与用PDGFS的随机胶原纤维相比，对齐的胶原蛋白-NP纤维显着促进了ADSC的理想初步分化，如肌腱蛋白和稀释剂等肌腱标记的增加程度所证明。另一方面，观察到通过不变的碱性磷酸酶和骨钙素测量的不希望的骨质发生分化。这些结果表明，对齐的胶原-NP复合纤维通过地形提示（对齐胶原纤维）和化学提示（从NPS释放的PDGF）诱导ADSC的遗传分化。因此，我们的新型对齐的胶原蛋白-NP复合纤维具有用于肌腱组织工程和再生的显着潜力。

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  • 来源
    《Acta biomaterialia》 |2014年第3期|共10页
  • 作者

    ChengX.; TsaoC.; SylviaV.L.; CornetD.; NicolellaD.P.; BredbennerT.L.; ChristyR.J.;

  • 作者单位

    Microencapsulation and Nanomaterials Department Southwest Research Institute 6220 Culebra Rd San;

    Microencapsulation and Nanomaterials Department Southwest Research Institute 6220 Culebra Rd San;

    Department of Orthopedics University of Texas Health Science Center at San Antonio 7703 Floyd;

    Department of Orthopedics University of Texas Health Science Center at San Antonio 7703 Floyd;

    Materials Engineering Department Southwest Research Institute 6220 Culebra Rd San Antonio TX;

    Materials Engineering Department Southwest Research Institute 6220 Culebra Rd San Antonio TX;

    Extremity Trauma and Regenerative Medicine Program US Army Institute of Surgical Research 3698;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 普通生物学;
  • 关键词

    Collagen; Growth Factors; Nanoparticle; Stem cell; Tendon;

    机译：胶原蛋白;生长因子;纳米粒子;干细胞;肌腱;

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