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首页> 外文期刊>Acta biomaterialia >Jellyfish collagen scaffolds for cartilage tissue engineering.
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Jellyfish collagen scaffolds for cartilage tissue engineering.

机译：软骨组织工程的水母胶原蛋白脚手架。

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摘要

Porous scaffolds were engineered from refibrillized collagen of the jellyfish Rhopilema esculentum for potential application in cartilage regeneration. The influence of collagen concentration, salinity and temperature on fibril formation was evaluated by turbidity measurements and quantification of fibrillized collagen. The formation of collagen fibrils with a typical banding pattern was confirmed by atomic force microscopy and transmission electron microscopy analysis. Porous scaffolds from jellyfish collagen, refibrillized under optimized conditions, were fabricated by freeze-drying and subsequent chemical cross-linking. Scaffolds possessed an open porosity of 98.2%. The samples were stable under cyclic compression and displayed an elastic behavior. Cytotoxicity tests with human mesenchymal stem cells (hMSCs) did not reveal any cytotoxic effects of the material. Chondrogenic markers SOX9, collagen II and aggrecan were upregulated in direct cultures of hMSCs upon chondrogenic stimulation. The formation of typical extracellular matrix components was further confirmed by quantification of sulfated glycosaminoglycans.
机译：多孔支架从水母Rhopilema Esculentum的精制胶原制成，用于软骨再生的潜在应用。通过浊度测量和原纤化胶原的定量评价胶原浓度，盐度和温度对原纤维形成的影响。通过原子力显微镜和透射电子显微镜分析确认具有典型带状图案的胶原型原纤维。通过冷冻干燥和随后的化学交联，制造来自水母胶原蛋白的多孔支架，从优化条件下精炼，并通过冷冻干燥和随后的化学交联制造。支架具有98.2％的开放性孔隙率。样品在循环压缩下稳定并显示出弹性行为。具有人间充质干细胞（HMSCs）的细胞毒性试验没有揭示材料的任何细胞毒性作用。软骨形成标志物SOX9，胶原蛋白II和蛋白在软骨形成刺激后的HMSCs直接培养上上调。通过定量硫酸化糖胺聚糖进一步证实典型细胞外基质组分的形成。

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