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首页> 外文期刊>Acta biomaterialia >Enzymatic pH control for biomimetic deposition of calcium phosphate coatings.
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Enzymatic pH control for biomimetic deposition of calcium phosphate coatings.

机译：磷酸钙涂层仿真沉积酶pH控制。

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摘要

The current study examines the enzymatic decomposition of urea into carbon dioxide and ammonia as a means to increase the pH during biomimetic deposition of calcium phosphate (CaP) onto implant surfaces. The kinetics of the enzymatically induced pH increase were studied by monitoring pH, calcium concentration and conductivity of the aqueous solutions as a function of time, urease concentration and initial concentrations of calcium and phosphate ions. Cryogenic transmission electron microscopy was used to study the process of homogeneous CaP precipitation in solution, whereas CaP deposition on conventional acid-etched titanium and micropatterned polystyrene (PS) surfaces was studied using scanning electron microscopy. The data presented in this study confirm that the substrate-enzyme combination urea-urease offers strong control over the rate of pH increase by varying the concentrations of precursor salts and urease. Formation of biomimetic CaP coatings was shown to proceed via formation of ionic polymeric assemblies of prenucleation complexes. The process of deposition and corresponding coating morphology was strongly dependent on the concentration of calcium, phosphate and urease. Finally, it was shown that the substrate-enzyme combination urea-urease allowed for spatial distribution of CaP crystals along the grooves of micropatterned PS surfaces at low concentrations of calcium, phosphate and urease, stressing the sensitivity of the presented method.
机译：目前的研究将尿素分解成二氧化碳和氨作为一种方法，作为将磷酸钙（帽）磷酸钙（帽）染色沉积在植入物表面上的手段。通过监测水溶液的pH，钙浓度和磷酸钙离子的函数，通过监测水溶液的pH，钙浓度和电导率来研究酶促诱导的pH增加的动力学。使用扫描电子显微镜研究了低温透射电子显微镜研究溶液中均匀帽沉淀的过程，而在常规酸蚀刻钛和微透明的聚苯乙烯（PS）表面上进行帽沉积。本研究中呈现的数据证实，通过改变前体盐和脲酶的浓度，基材 - 酶组合尿素脲提供了对pH增加的强烈控制。显示了仿真帽涂层的形成，通过形成预核复合物的离子聚合物组装。沉积和相应的涂层形态的方法强烈依赖于钙，磷酸盐和脲酶的浓度。最后，表明，基质 - 酶组合尿素脲脲允许在低浓度的钙，磷酸盐和脲酶的微透明理由的PS表面的凹槽中允许帽晶体的空间分布，强调所示方法的灵敏度。

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