首页> 外文期刊>Acta biomaterialia >A novel carboxymethyl chitosan-quantum dot-based intracellular probe for Zn2+ ion sensing in prostate cancer cells.
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A novel carboxymethyl chitosan-quantum dot-based intracellular probe for Zn2+ ion sensing in prostate cancer cells.

机译:前列腺癌细胞中Zn2 +离子传感的一种新型羧甲基壳聚糖 - 量子点细胞内探针。

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In this paper, we fabricated novel carboxymethyl chitosan-coated CdTe quantum dots (CMC-CdTe QDs) via the electrostatic interaction between amino groups in the carboxymethyl chitosan polymeric chains and carboxyl groups of the CdTe QDs. Carboxymethyl chitosan on the surface of CdTe QDs had strong binding ability with Zn(2+), resulting in the obvious enhancement of the photoluminescence of CdTe QDs. The photoluminescence intensity of CMC-CdTe QDs probe was proportional to the concentration of Zn(2+) in the range of 5.0 × 10(-6) to 5.0 × 10(-3) mol l(-1). The detection limit for Zn(2+) was 4.5 × 10(-6) mol l(-1). The experimental results indicate that the CMC-CdTe QDs possess favorable cell compatibility, good sensitivity and selectivity for intracellular Zn(2+) sensing, and are promising candidates for cellular imaging and sensing in prostate cancer cells. The present study also provides an approach for the further development of nanoprobes dedicated to intracellular sensing.
机译:在本文中,我们通过羧甲基壳聚糖聚合物链中的氨基之间的静电相互作用制造了新的羧甲基壳聚糖涂覆的CDTE量子点(CMC-CDTE QDS),CDTE QDS的羧基。 CdTe QD表面上的羧甲基壳聚糖具有较强的结合能力与Zn(2+),导致CdTe QD的光致发光的明显增强。 CMC-CDTE QDS探针的光致发光强度与5.0×10(-6)至5.0×10(-3)摩尔L(-1)的Zn(2+)的浓度成比例。 Zn(2+)的检测限为4.5×10(-6)mol L(-1)。 实验结果表明,CMC-CDTE QD具有良好的细胞相容性,对细胞内Zn(2+)感测的良好敏感性和选择性,并且是前列腺癌细胞中的细胞成像和感应的候选者。 本研究还提供了一种方法,用于进一步发展纳米熟体,致力于细胞内感测。

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