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首页> 外文期刊>Acta biomaterialia >Post microtextures accelerate cell proliferation and osteogenesis.
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Post microtextures accelerate cell proliferation and osteogenesis.

机译：后微纹理加速细胞增殖和骨质发生。

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摘要

The influence of surface microtexture on osteogenesis was investigated in vitro by examining the proliferation and differentiation characteristics of a class of adult stem cells and their progeny, collectively known as connective tissue progenitor cells (CTPs). Human bone marrow-derived CTPs were cultured for up to 60 days on smooth polydimethylsiloxane (PDMS) surfaces and on PDMS with post microtextures that were 10 microm in diameter and 6 microm in height, with 10 microm separation. DNA quantification revealed that the numbers of CTPs initially attached to both substrates were similar. However, cells on microtextured PDMS transitioned from lag phase after 4 days of culture, in contrast to 6 days for cells on smooth surfaces. By day 9 cells on the smooth surfaces exhibited arbitrary flattened shapes and migrated without any preferred orientation. In contrast, cells on the microtextured PDMS grew along the array of posts in an orthogonal manner. By days 30 and 60 cells grew and covered all surfaces with extracellular matrix. Western blot analysis revealed that the expression of integrin alpha5 was greater on the microtextured PDMS compared with smooth surfaces. Real time reverse transcription-polymerase chain reaction revealed that gene expression of alkaline phosphatase had decreased by days 30 and 60, compared with that on day 9, for both substrates. Gene expression of collagen I and osteocalcin was consistently greater on post microtextures relative to smooth surfaces at all time points.
机译：通过检查一类成年干细胞的增殖和分化特性及其后代，共同称为结缔组织祖细胞（CTPS），在体外研究表面微纹理对骨发生的影响。在光滑的聚二甲基硅氧烷（PDMS）表面和具有10微米直径和6微米的PDMS上培养60天的人骨髓衍生的CTP培养至多60天，高度为10微米，高度为6微米，分离10微米。 DNA定量揭示最初连接到两个基材上的CTP的数量相似。然而，在培养4天后的微调PDM上的细胞从培养后4天后转变为6天，在光滑表面上的细胞6天。在光滑表面上的第9天的9个细胞表现出任意扁平的形状并没有任何优选的方向迁移。相反，微织狭窄的PDM上的细胞沿着正交方式沿着柱子阵列增长。 30天和60个细胞增长并覆盖了细胞外基质的所有表面。 Western印迹分析显示，与光滑表面相比，整合蛋白α5的表达更大。实时逆转录 - 聚合酶链反应显示，与第9天相比，碱性磷酸酶的基因表达在30天和60天，两种底物。胶原I和骨钙素的基因表达在微调上相对于所有时间点的光滑表面始终如一。

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