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首页> 外文期刊>Acta neurobiologiae experimentalis >BDNF expression in cat striate cortex is regulated by binocular pattern deprivation
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BDNF expression in cat striate cortex is regulated by binocular pattern deprivation

机译：Cat Strative Cortex中的BDNF表达受双目模式剥夺的管制

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摘要

Deprivation of patterned visual information, as in early onset congenital cataract patients, results in a severe impairment in global motion perception. Previously we reported a delayed maturation of the peripheral visual field representation in primary visual area 17, based on a 2-D DIGE screen for protein expression changes and in situ hybridization for the activity reporter gene ZIF268. To corroborate these findings we here explore the binocular pattern deprivation (BD)-regulated expression of brain-derived neurotrophic factor (BDNF), a well-described neurotrophin precipitously regulated by early visual experience. To assess the timing of maturation-related BDNF expression we compared the central and the peripheral visual field representations of area 17 of 1, 2, 4 and 6-month-old and adult cats reared under normal visual conditions. To scrutinize the outcome of BD, four different deprivation strategies were compared, including early onset BD from birth and lasting for 2, 4 or 6 months (2BD, 4BD, 6BD), and late onset BD for 2 months upon 2 months of normal vision (2N2BD), as animal models of congenital and delayed onset cataract. During normal cortical development the BDNF transcript levels, measured by quantitative RT-PCR, remained stable. Higher BDNF mRNA levels were found in central area 17 of 2BD and 6BD animals compared to age-matched controls. In central area 17, the high BDNF mRNA levels at the end of the BD period may activate a mechanism by which plastic processes, halted by deprivation, may begin. We here confirm that the peripheral visual field representation of area 17 matures slower than its central counterpart. Only in central area 17 normal visual input upon BD could upregulate BDNF mRNA which may lead to a fast activation of local plastic adaptations.
机译：如早期发作先天性白内障患者，剥夺了图案的视觉信息，导致全球运动感知的严重损害。此前，我们报告了基于蛋白质表达的2-D Dige筛网的主要视觉区域17中的外周视场表示的延迟成熟，用于蛋白质表达的变化和用于活动报告基因ZIF268的原位杂交。为了证实这些发现，我们在这里探讨双目模式剥夺（BD） - 通过早期视觉体验急于调节脑衍生的神经营养因子（BDNF）的脑衍生的神经营养因子（BDNF）的表达。为了评估与在正常视觉条件下饲养的1,2,4和6个月的区域17的中央和周边视野表示的中央和周边视野表示的时间和周边视野表示。为了仔细检查BD的结果，比较了四种不同的剥夺策略，包括从出生的早期发病BD，持续2,4或6个月（2bd，4bd，6bd），并且在正常视觉的2个月后2个月晚期发作2个月（2N2BD），作为先天性和延迟发作白内障的动物模型。在正常皮质发育过程中，通过定量RT-PCR测量的BDNF转录物水平保持稳定。与年龄匹配的对照相比，在2BD和6BD动物的中心区域中发现了较高的BDNF mRNA水平。在中心区域17中，BD时段结束时的高BDNF mRNA水平可以激活塑料过程，通过剥夺停止停止的机制，可以开始。我们在此确认区域17的外围视野表示比其中心对应慢。仅在中心区域17上，BD上的正常视觉输入可以上调BDNF mRNA，这可能导致局部塑料适应的快速激活。

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