...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Crystal growth & design >Flash cooling protein crystals: Estimate of cryoprotectant concentration using thermal properties
【24h】

Flash cooling protein crystals: Estimate of cryoprotectant concentration using thermal properties

机译：闪蒸冷却的蛋白质晶体：使用热性质估算防冻剂浓度

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

X-ray diffraction from protein crystals is routinely measured at cryogenic temperatures, primarily to minimize radiation damage. Protein crystals and the surrounding mother liquor have high water content, which can lead to ice formation when samples are cooled to cryogenic temperatures. Cryoprotectants are added to the aqueous mother liquor solutions to achieve both vitreous water and to retain protein crystal integrity. Finding a minimum cryoprotectant concentration to avoid ice formation is a trial and error procedure, and valuable crystals are often lost in this exercise. To minimize crystal loss, a predictive algorithm for estimation of cryoprotectant concentration requirements for successful flash cooling was developed based on heat transfer analysis and thermal properties of cryoprotectant solutions. These thermal properties such as glass transition temperature, melting temperature, and specific heat capacity were measured and modeled for aqueous glycerol and ethylene glycol solutions, and ternary mixtures of glycerol-salt-water. The minimum amounts of cryoprotectant required for successful flash cooling in samples of varying size were evaluated by X-ray diffraction, and a target cooling time for sample vitrification was identified. Apredictive heat transfer analysis and property algorithm for cryoprotectant requirements was verified for neutron diffraction sized crystals of D-xylose isomerase and Aeropyrum pernix flap endonuclease 1.
机译：通常在低温下测量蛋白质晶体的X射线衍射，主要是为了最大程度地减少辐射损伤。蛋白质晶体和周围的母液中水含量高，当样品冷却至低温时会导致结冰。将低温保护剂添加到母液水溶液中，以同时获得玻璃水和保持蛋白质晶体的完整性。找到最低的防冻剂浓度以避免结冰是一个反复试验的过程，在此练习中经常会丢失有价值的晶体。为了最大程度地减少晶体损失，基于热传递分析和冷冻保护剂溶液的热性质，开发了一种预测算法，用于估算成功完成快速冷却所需的冷冻保护剂浓度。测量并模拟了甘油和乙二醇水溶液以及甘油-盐-水的三元混合物的这些热性能，例如玻璃化转变温度，熔融温度和比热容。通过X射线衍射评估了不同大小的样品中成功闪速冷却所需的最小量的防冻剂，并确定了样品玻璃化的目标冷却时间。对D-木糖异构酶和Aeropyrum多年生皮瓣内切核酸酶1的中子衍射尺寸晶体进行了预测性传热分析和防冻剂要求的性能算法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号