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Biocompatibility assessment of a long-term wearable artificial pump-lung in sheep

机译：生物相容性评估绵羊中长期可穿戴人工泵肺的分析

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摘要

The purpose of this study was to assess the biocompatibility of a newly developed long-term wearable artificial pump-lung (APL) in a clinically relevant ovine animal model. The wearable APL device was implanted in five sheep through left thoracotomy. The device was connected between the right atrium and pulmonary artery and evaluated for 30 days. Three sheep were used as the sham control. Platelet activation was assessed by measuring platelet surface P-selectin (CD62P) expression with flow cytometry and plasma soluble P-selectin with an enzyme-linked immunosorbent assay. Thrombotic deposition on the device components and hollow fiber membranes were analyzed with digital imaging and scanning electron microscopy. Surface P-selectin of the APL and sham groups changed significantly over the study period, but without significant differences between the two groups. Soluble P-selectin for the two groups peaked in the first 24h after the surgery. Soluble P-selectin of the APL group remained slightly elevated over the study period compared to the presurgical baseline value and was slightly higher compared to that of the sham group. Plasma free hemoglobin remained in the normal ranges in all the animals. In spite of the surgery-related alteration in laboratory tests and elevation of platelet activation status, the APL devices in all the animals functioned normally (oxygen transfer and blood pumping) during the 30-day study period. The device flow path and membrane surface were free of gross thrombus. Electron microscopy images showed only scattered thrombi on the fibers (membrane surface and weft). In summary, the APL exhibited excellent biocompatibility. Two forms of platelet activation, surgery-related and device-induced, in the animals implanted with the wearable APL were observed. The limited device-induced platelet activation did not cause gross thrombosis and impair the long-term device performance.
机译：本研究的目的是在临床相关的绵羊动物模型中评估新开发的长期可穿戴人工泵肺（APL）的生物相容性。可穿戴APL器件通过左胸传道术植入五只绵羊中。该装置连接在右心房和肺动脉之间，并评估30天。三只羊被用作假控制。通过用流式细胞术测定血小板表面p-选择蛋白（CD62P）表达和具有酶联免疫吸附测定的血小板表面p-选择蛋白（CD62P）表达来评估血小板活化。用数字成像和扫描电子显微镜分析装置组分和中空纤维膜上的血栓形成沉积。 APL和假组的表面p-Selectin在研究期间显着改变，但两组之间没有显着差异。用于两组的可溶性p-Selectin在手术后的前24小时达到峰值。与前尿基基线值相比，APL组的可溶性P-选择蛋白在研究期间略微升高，与假群相比略高。血浆免血红蛋白仍然是所有动物的正常范围。尽管在实验室测试和血小板激活状态的升高中进行了与外科有关的改变，但在30天的研究期间，所有动物的APL器件在所有动物中正常运行（氧气转移和血液泵送）。器件流动路径和膜表面没有总血栓。电子显微镜图像仅显示纤维（膜表面和纬纱）上的散射血栓。总之，APL表现出优异的生物相容性。观察到植入可穿戴APL的动物的两种形式的血小板激活，手术相关和设备诱导。有限的装置诱导的血小板活化不会导致血栓形成和损害长期设备性能。

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  • 来源
    《Artificial Organs》 |2013年第8期|共11页
  • 作者

    ZhouK.; NiuS.; BianchiG.; WeiX.; GarimellaN.; GriffithB.P.; WuZ.J.;

  • 作者单位

    Artificial Organs Laboratory Department of Surgery University of Maryland School of Medicine;

    Artificial Organs Laboratory Department of Surgery University of Maryland School of Medicine;

    Artificial Organs Laboratory Department of Surgery University of Maryland School of Medicine;

    Artificial Organs Laboratory Department of Surgery University of Maryland School of Medicine;

    Artificial Organs Laboratory Department of Surgery University of Maryland School of Medicine;

    Artificial Organs Laboratory Department of Surgery University of Maryland School of Medicine;

    Artificial Organs Laboratory Department of Surgery University of Maryland School of Medicine;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 器官移植术;
  • 关键词

    Artificial lungs; Biocompatibility; Extracorporeal membrane oxygenation; Integrated pump-oxygenators; Platelet activation;

    机译：人工肺;生物相容性;体外膜氧合;集成泵 - 氧气器;血小板激活;

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