Nucleotide sequence and deduced amino acid sequence of a putative asparagine synthetase in the mosquito Aedes aegypti (L.)

Urs Ackermann; Rolf Graf

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Nucleotide sequence and deduced amino acid sequence of a putative asparagine synthetase in the mosquito Aedes aegypti (L.)

机译：埃及伊蚊（L.）中推定的天冬酰胺合成酶的核苷酸序列和推导的氨基酸序列

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摘要

A cDNA was cloned from a Aedes aegypti head cDNA library, containing the complete coding sequence for an asparagine synthetase homolog. The predicted polypeptide sequence exhibits high homology with different proteins of the 'purF' glutamine amidotransferase enzyme family. The aminoterminal region, containing Cys-1 which is crucial to perform the glutaminase reaction, was highly conserved among the asparagine synthetase family. Subsequent expression of the cDNA yielded a 54 000 Da protein corresponding to the molecular weight of other asparagine synthetases.

机译：从埃及伊蚊头cDNA文库中克隆一个cDNA，其中包含天冬酰胺合成酶同源物的完整编码序列。预测的多肽序列与“ purF”谷氨酰胺酰胺转移酶家族的不同蛋白质具有高度同源性。在天冬酰胺合成酶家族中，含有对谷氨酰胺酶反应至关重要的Cys-1的氨基末端区域高度保守。 cDNA的后续表达产生了一个54 000 Da的蛋白质，该蛋白质与其他天冬酰胺合成酶的分子量相对应。

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《Biochimica et Biophysica Acta. Protein Structure and Molecular Enzymology》 |1998年第0期|共4页
作者
Urs Ackermann; Rolf Graf;
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正文语种 eng
中图分类生物化学;分子生物学;
关键词
'purF' glutamine amidotransferase; glutaminase activity; asparagine synthesis; cDNA sequence; cDNA expression; baculovirus expression system;

机译：“ purF”谷氨酰胺酰胺转移酶;谷氨酰胺酶活性;天冬酰胺合成;cDNA序列;cDNA表达;杆状病毒表达系统;

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1. Nucleotide sequence and deduced amino acid sequence of a putative asparagine synthetase in the mosquito Aedes aegypti (L.). [J] . Ackermann U, Graf R Biochimica et biophysica acta: international journal of biochemistry and biophysics . 1998,第2期

机译：埃及伊蚊（L.）中推定的天冬酰胺合成酶的核苷酸序列和推导的氨基酸序列。
2. Amino acid sequence of a putative transposase protein of the medaka fish transposable element Tol2 deduced from mRNA nucleotide sequences [J] . Hori Hiroshi, Koga Akihiko, Maruyama Yuki, FEBS Letters . 1999,第3期

机译：从mRNA核苷酸序列推论med鱼转座因子Tol2推定转座酶蛋白的氨基酸序列
3. A New York isolate of Soil-borne wheat mosaic virus differs considerably from the Nebraska type strain in the nucleotide sequences of various coding regions but not in the deduced amino acid sequences. [J] . Koenig R ba.de, Bergstrom GC, Gray SM, Archives of virology . 2002,第3期

机译：纽约分离的土壤传播的小麦花叶病毒与内布拉斯加州型菌株在各个编码区的核苷酸序列上有很大不同，但在推导的氨基酸序列上却没有很大不同。
4. New insights on the fragmentation of protonated amino acids involved in ammonia metabolism in Aedes aegypti mosquitoes [C] . Xin Ma, Shai Dagan, Arpad Somogyi, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：对氨氨蚊虫氨代谢涉及氨代谢的质子化氨基酸片段的新见解
5. Development of a diagnostic method to allow strain-level identification of Staphylococcus aureus based on the nucleotide sequence of the enterotoxin gene and the amino acid sequence of its enterotoxin [D] . James, Mindy M. 2008

机译：根据肠毒素基因的核苷酸序列及其肠毒素的氨基酸序列，开发一种诊断方法，以菌株级鉴定金黄色葡萄球菌
6. Complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L. An abundant transcript induced by transformation of fibroblasts. [O] . L J Joseph, L C Chang, D Stamenkovich, 1988

机译：人和鼠原蛋白酶的完整核苷酸和推导氨基酸序列。成纤维细胞转化诱导的丰富转录本。
7. Amino acid sequence of a putative transposase protein of the medaka fish transposable element Tol2 deduced from mRNA nucleotide sequences [O] . Koga Akihiko, Suzuki Miho, Maruyama Yuki, 1999

机译：从mRNA核苷酸序列推论med鱼转座因子Tol2推定转座酶蛋白的氨基酸序列

Nucleotide sequence and deduced amino acid sequence of a putative asparagine synthetase in the mosquito Aedes aegypti (L.)

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