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首页> 外文期刊>International immunopharmacology >Ginsenoside Rg3 promotes Fc gamma receptor-mediated phagocytosis of bacteria by macrophages via an extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase-dependent mechanism
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Ginsenoside Rg3 promotes Fc gamma receptor-mediated phagocytosis of bacteria by macrophages via an extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase-dependent mechanism

机译：人参皂甙RG3通过细胞外信号调节激酶1/2和P38丝裂原激活的蛋白激酶依赖性机制促进巨噬细胞的FCγ受体介导的细菌吞噬作用

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摘要

Ginsenoside Rg3 is a steroidal saponin abundant in Korean red ginseng that has high anti-inflammatory activity. Rg3 exerts an immunomodulatory effect in acute inflammatory conditions such as bacterial infections. In this study, we determined the effect of Rg3 on bacterial uptake by macrophages and the related intracellular signaling pathways. Rg3 increased macrophage phagocytosis of IgG-opsonized Escherichia coli and IgG-opsonized beads (IgGbeads), but not of non-opsonized beads. Rg3 also enhanced the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 and p38 mitogen-activated protein kinase (p38 MAPK), but not that of Akt. The inclusion of IgGbeads in macrophage cultures also increased the phosphorylation of ERK1/2 and p38, but co-culture of macrophages with non-opsonized beads did not affect the phosphorylation of ERK1/2 and p38. The Rg3-induced promotion of phagocytosis was inhibited by PD98059, an ERK1/2 inhibitor, and SB203580, a p38 inhibitor. PD98059 inhibited Rg3-induced p38 MAPK phosphorylation, but SB203580 did not suppress ERK1/2 phosphorylation. Culture of macrophages with Rg3 increased actin polymerization, and this effect was inhibited by SB203580 and PD98059. The Rg3-induced increase in phagocytosis was also inhibited by NSC23766, a Rac1 inhibitor and CASIN, a Cdc42 inhibitor. Intraperitoneal injection of Rg3 increased the phosphorylation of ERK1/2 and p38 as well as the phagocytosis of bacteria by lung cells. These results demonstrate that ginsenoside Rg3 enhances macrophage phagocytosis of bacteria by activating the ERK1/2 and p38 MAPK pathways.
机译：人参皂苷Rg3是韩国红人参丰富的甾体皂苷，具有高抗炎活动。 RG3在急性炎症条件下施加免疫调节作用，例如细菌感染。在这项研究中，我们确定RG3对巨噬细胞和相关细胞内信号传导途径对细菌摄取的影响。 RG3增加了IgG-Opsonize大肠杆菌和IgG-Opsonize珠粒（IgGBeads）的巨噬细胞吞噬作用，但不是非透镜化珠子。 RG3还增强了细胞外信号调节激酶（ERK）1/2和P38丝裂原激活蛋白激酶（P38 MAPK）的磷酸化，但不是AKT的磷酸化。在巨噬细胞培养物中包含IgGBeads也增加了ERK1 / 2和P38的磷酸化，但巨噬细胞的巨噬细胞的共培养不影响ERK1 / 2和P38的磷酸化。通过PD98059，ERK1 / 2抑制剂和SB203580，P38抑制剂抑制RG3诱导的吞噬作用促进。 PD98059抑制RG3诱导的P38 MAPK磷酸化，但SB203580没有抑制ERK1 / 2磷酸化。 RG3的巨噬细胞培养增加增加肌动蛋白聚合，SB203580和PD98059抑制了这种效果。 NSC23766，RAC1抑制剂和CASIN，CDC42抑制剂也抑制了RG3诱导的吞噬作用的增加。腹膜内注射RG3增加了ERK1 / 2和P38的磷酸化以及肺细胞吞噬细菌的吞噬作用。这些结果表明，人参皂甙RG3通过激活ERK1 / 2和P38 MAPK途径来增强细菌的巨噬细胞吞噬作用。

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