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The Ciona intestinalis cleavage clock is independent of DNA methylation

机译：Ciona intestinalis切割时钟与DNA甲基化无关

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The initiation of embryonic gene expression in ascidian embryos appears to be tightly regulated by the number of DNA replication cycles. DNA methylation is thought to contribute to the clock mechanism that counts the rounds of DNA replication. We used mass spectrometry and whole genome bisulfite sequencing to characterize DNA methylation changes that occur in early developmental stages of the ascidian, Ciona intestinalis. We found that global DNA methylation in early Ciona development was static, and a base-wise comparison between the genomes of consecutive developmental stages found no DNA demethylation that was related to zygotic gene activation. Additionally, 5hmC was hardly detected by mass spectrometry in the developmental samples, suggesting a lack of demethylation mediated by ten eleven translocation (TET) methylcytosine dioxygenase in C. intestinalis. We conclude that DNA methylation is not involved in regulating DNA replication-dependent transcriptional activation. (C) 2016 Elsevier Inc. All rights reserved.
机译：在阿立西尼亚胚胎中胚胎基因表达的开始似乎受到DNA复制循环的数量的紧密调节。据认为，DNA甲基化有助于计算DNA复制的轮流的时钟机制。我们使用了质谱和全基因组亚硫酸氢盐测序，以表征在阿立西安肝癌早期发育阶段发生的DNA甲基化变化。我们发现早期癌原发育中的全球DNA甲基化是静态的，并且连续发育阶段的基因组之间的基本比较发现没有与Zygotic Gene活化有关的DNA去甲基化。另外，在发育样品中，质谱法几乎不检测到5HMC，表明在C. intestinalis中缺乏由十个易位（TET）甲基胞嘧啶二氧基酶的1011级易位（TET）甲基胞嘧啶二氧化酶介导的去甲基化。我们得出结论，DNA甲基化不参与调节DNA复制依赖性转录活化。 （c）2016年Elsevier Inc.保留所有权利。

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