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首页> 外文期刊>Genomics >Transcriptome analysis reveals potential genes involved in flower pigmentation in a red-flowered mutant of white clover (Trifolium repens L.)
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Transcriptome analysis reveals potential genes involved in flower pigmentation in a red-flowered mutant of white clover (Trifolium repens L.)

机译：转录组分析揭示了白三叶草红花突变体中的花色素沉着潜在的基因（三种repens L.）

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摘要

White clover (Trifolium repens L.) has been cultivated for ornamental use because of its flowers, leaf marks and creeping habit. Although a mutation in flower color is very infrequent in this species, the red-flowered mutant of white clover was a novel germplasm for ornamental white clover breeding. The mechanism of flower pigmentation in white clover is still limited because of the rarity of mutation materials and the lack of genomic data. In this study, two cDNA libraries from red-flowered white clover mutant between sunlight-exposed plants and shade-treated plants, respectively, were used for transcriptome sequencing. A total of 157,964 unigenes with an average length of 728 bp and a median length of 1346 bp were isolated. A large number of differentially expressed genes (6282) that were potentially involved in multiple biological and metabolic pathways, including anthocyanin flavonoid biosynthetic pathway and flavonoid biosynthetic pathway, were obtained, 70 of which could be identified as putative homologues of color-related genes. Furthermore, eight key candidate genes (CHS, F3'H, F3'5'H, UFGT, FLS, LAR, ANS, and DFR) in flavonoid biological synthesis pathway were identified by quantitative real-time PCR (qRT-PCR). Mass sequence data obtained by RNA-Seq of white clover and its red-flowered mutant provided basic sequence information and a platform for future molecular biological research on the red flower trait.
机译：由于其花，叶标和匍匐习惯，为装饰用途培养了白色三叶草（三梨糖repens L.）。虽然在这种物种中，花色中的突变非常罕见，但白三叶草的红花突变体是一种用于装饰白三叶草繁殖的新种质。由于突变材料的罕见和缺乏基因组数据，白三叶草花色素沉着的机制仍然有限。在本研究中，用于分别在阳光暴露的植物和阴影处理植物之间的红花白三叶草突变体的两个cDNA文库用于转录组测序。分离总长度为728bp的157,964个ungenenes和1346bp的中值长度。获得了大量差异表达的基因（6282），其潜在参与多种生物和代谢途径，包括花青素类黄酮生物合成途径和类黄酮化生物合成途径，其中70个可以被鉴定为彩色相关基因的推定同源物。此外，通过定量实时PCR（QRT-PCR）鉴定了在类黄酮生物合成途径中的八个关键候选基因（CHS，F3'H，F3'5'H，UFGT，FLS，LAR，ANS和DFR）。由白色三叶草的RNA-SEQ获得的质量序列数据和其红花突变体提供了基本序列信息和未来分子生物研究的平台，用于红色花特征。

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  • 来源
    《Genomics》 |2018年第3期|共10页
  • 作者

    Zhang Heshan; Tian Hong; Chen Mingxin; Xiong Junbo; Cai Hua; Liu Yang;

  • 作者单位

    Hubei Acad Agr Sci Inst Anim Husb &

    Vet Sci Hubei Key Lab Anim Embryo &

    Mol Breeding Wuhan;

    Hubei Acad Agr Sci Inst Anim Husb &

    Vet Sci Hubei Key Lab Anim Embryo &

    Mol Breeding Wuhan;

    Hubei Acad Agr Sci Inst Anim Husb &

    Vet Sci Hubei Key Lab Anim Embryo &

    Mol Breeding Wuhan;

    Hubei Acad Agr Sci Inst Anim Husb &

    Vet Sci Hubei Key Lab Anim Embryo &

    Mol Breeding Wuhan;

    Hubei Acad Agr Sci Inst Anim Husb &

    Vet Sci Hubei Key Lab Anim Embryo &

    Mol Breeding Wuhan;

    Hubei Acad Agr Sci Inst Anim Husb &

    Vet Sci Hubei Key Lab Anim Embryo &

    Mol Breeding Wuhan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学遗传学;
  • 关键词

    Flower pigmentation; Trifolium repens; Transcriptome analysis; Red-flowered mutant;

    机译：花卉色素沉着;三粒重复;转录组分析;红花突变体;

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