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Bacterial Community of Koumiss from Mongolia Investigated by Culture and Culture-Independent Methods

机译：蒙古族的koumiss细菌群落调查文化和文化无关的方法

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摘要

The bacterial community of fermented horse milk (koumiss) from Mongolia was studied using three methods: cultivation, direct identification by 16S rRNA clone library and denaturing gradient gel electrophoresis (DGGE). Ninety-eight strains were isolated by traditional cultivation and 61 of those were randomly selected for further identification by 16S rRNA gene sequencing. The strains were dominated by lactic acid bacteria (LAB; six different lactobacilli), Acinetobacter, Bacillus and Psychrobacter. Construction of the clone library analysis revealed that 16S sequences of 220 clones, genus Lactobacillus was dominant, but Streptococcus thermophilus, Acetobacter pasteurianus and uncultured clones were also detected. Ten unique bands were sequenced from the DGGE and revealed: Lactococcus lactis, Lactococcus lactis subsp. lactis, Clostridium acidurici, Acinetobacter johnsonii, Dickeya sp., Enterobacter sp., Pseudomonas sp., Raoultella sp., and Ruminococcus sp.. In vitro growth inhibition of three human pathogens, Escherichia coli, Enterobacter sakazakii and Staphylococcus aureus by 14 culturable bacteria displayed that only three of the isolates tested inhibit growth of E. sakazakii while most of the other bacteria delayed growth of the target bacteria.
机译：使用三种方法研究了来自蒙古的发酵马牛奶（Koumiss）的细菌群落：培养，直接鉴定16S rRNA克隆文库和变性梯度凝胶电泳（DGGE）。通过传统培养分离98株菌株，随机选择61种，以进一步鉴定16S rRNA基因测序。菌株由乳酸菌（实验室;六种不同的乳杆菌），雌激素，芽孢杆菌和心理杆菌占主导地位。克隆文库分析的构建揭示了16S序列的220个克隆，乳杆菌属占优势，但也检测到嗜热链球菌，乙腺脊髓植物和未培养的克隆。从DGGE测序十个独特的乐谱，透露：乳酸乳球菌，乳酸乳酸乳酸乳糜蛋白。乳酸乳裂，酸菌，johnsonii，dickekss sp。，肠杆菌sp。，endomonas sp。，raoultella sp。，Raoultella sp。和喇菇的三种人类病原体的体外生长抑制，14种培养细菌的14种培养基显示只有三个分离株测试抑制E. Sakazakii的生长，而大多数其他细菌会延迟靶细菌的生长。

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