Molecular evidence for an intrinsic circadian pacemaker in the cardiac ganglion of the American lobster, Homarus americanus - Is diel cycling of heartbeat frequency controlled by a peripheral clock system ?

Christie Andrew E.; Yu Andy; Roncalli Vittoria; Pascual Micah G.; Cieslak Matthew C.; Warner Amanda N.; Lameyer Tess J.; Stanhope Meredith E.; Dickinson Patsy S.; Hull J. Joe

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Molecular evidence for an intrinsic circadian pacemaker in the cardiac ganglion of the American lobster, Homarus americanus - Is diel cycling of heartbeat frequency controlled by a peripheral clock system ?

机译：在美国龙虾的心神经神经节中，仿木族的内在昼夜节目起搏器的分子证据 - 是由外围时钟系统控制的心跳频率的Diel循环吗？

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Whether cardiac output in decapod crustaceans is under circadian control has long been debated, with mixed evidence for and against the hypothesis. Moreover, the locus of the clock system controlling cardiac activity, if it is under circadian control, is unknown. However, a report that the crayfish heart in organ culture maintains a circadian oscillation in heartbeat frequency suggests the presence of a peripheral pacemaker within the cardiac neuromuscular system itself. Because the decapod heart is neurogenic, with contractions controlled by the five motor and four premotor neurons that make up the cardiac ganglion (CG), a likely locus for a circadian clock is the CG itself. Here, a CG-specific transcriptome was generated for the lobster, Homarus americanus, and was used to assess the presence/absence of transcripts encoding putative clock-related proteins in the ganglion. Using known Homarus brain/eyestalk ganglia clock-related proteins as queries, BLAST searches of the CG transcriptome were conducted for the five proteins that form the core clock, ie., clock, cryptochrome 2, cycle, period and timeless, as well as for a variety of clock-associated, clock input pathway and clock output pathway proteins. With the exception of pigment dispersing hormone receptor [PDHR], a putative clock output pathway protein, one or more transcripts encoding each of the proteins searched for were identified from the CG assembly; no PDHR-encoding transcripts were found. RT-PCR confirmed the expression of all core clock transcripts in multiple independent CG cDNAs; RNA-Seq data suggest that both the motor and premotor neurons could contribute to the cellular locus of a pacemaker. These data provide support for the possible existence of an intrinsic circadian clock in the H. americanus CG, and form a foundation for guiding future anatomical, molecular and physiological investigations of circadian signaling in the lobster cardiac neuromuscular system.

机译：在甲板上的心脏输出是否在昼夜控制下长期以来一直在争论，具有混合证据和反对假设。而且，控制心脏活性的时钟系统的基因座，如果是在昼夜控制下，是未知的。然而，一份报告称，器官文化中的小龙虾心脏在心跳频率上保持昼夜振荡，表明心脏神经肌肉系统本身内的外周起搏器存在。因为脱像心脏是神经源性的，所以通过五个电动机和四个紧球神经元来控制的收缩，核心神经节（CG），可能的昼夜节日的可能性是CG本身。这里，为龙虾，冠状疱疹生成CG特异性转录组，并且用于评估在神经节中编码推定的时钟相关蛋白的转录物的存在/不存在。使用已知的Homarus Brain / Eyeskalk Ganglia时钟时钟相关的蛋白作为查询，对CG转录组的Blast搜索是针对形成核心时钟的五种蛋白质，即，时钟，加密2，周期，周期和永恒，以及各种时钟相关，时钟输入通路和时钟输出路径蛋白。除了颜料分散激素受体[PDHR]，从CG组件中鉴定了调用时钟输出途径蛋白，编码每个搜索的每个蛋白质的转录物;没有找到PDHR编码的转录物。 RT-PCR确认了多个独立CG CDNA中所有核心钟转录物的表达; RNA-SEQ数据表明，电动机和热球神经元都可以有助于起搏器的细胞基因座。这些数据提供了支持H. Americanus CG中的内在昼夜昼夜昼夜时钟的支持，并形成龙虾心脏神经肌肉系统中昼夜节律信号传导的未来解剖学，分子和生理调查的基础。

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来源
《Marine genomics》 |2018年第2018期|共12页
作者
Christie Andrew E.; Yu Andy; Roncalli Vittoria; Pascual Micah G.; Cieslak Matthew C.; Warner Amanda N.; Lameyer Tess J.; Stanhope Meredith E.; Dickinson Patsy S.; Hull J. Joe;
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作者单位

Univ Hawaii Manoa Sch Ocean &

Earth Sci &

Technol Pacific Biosci Res Ctr Bekesy Lab Neurobiol 1993 East West Rd Honolulu HI 96822 USA;

Univ Hawaii Manoa Sch Ocean &

Earth Sci &

Technol Pacific Biosci Res Ctr Bekesy Lab Neurobiol 1993 East West Rd Honolulu HI 96822 USA;

Univ Hawaii Manoa Sch Ocean &

Earth Sci &

Technol Pacific Biosci Res Ctr Bekesy Lab Neurobiol 1993 East West Rd Honolulu HI 96822 USA;

Univ Hawaii Manoa Sch Ocean &

Earth Sci &

Technol Pacific Biosci Res Ctr Bekesy Lab Neurobiol 1993 East West Rd Honolulu HI 96822 USA;

Univ Hawaii Manoa Sch Ocean &

Earth Sci &

Technol Pacific Biosci Res Ctr Bekesy Lab Neurobiol 1993 East West Rd Honolulu HI 96822 USA;

USDA ARS US Arid Land Agr Res Ctr Pest Management &

Biocontrol Res Unit Maricopa AZ 85138 USA;

Bowdoin Coll Dept Biol 6500 Coll Stn Brunswick ME 04672 USA;

Bowdoin Coll Dept Biol 6500 Coll Stn Brunswick ME 04672 USA;

Bowdoin Coll Dept Biol 6500 Coll Stn Brunswick ME 04672 USA;

USDA ARS US Arid Land Agr Res Ctr Pest Management &

Biocontrol Res Unit Maricopa AZ 85138 USA;

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原文格式 PDF
正文语种 eng
中图分类遗传学;
关键词
Biological rhythm; Circadian rhythm; Transcriptomics; Crustacea; Decapoda; Cardiac output;

机译：生物节奏;昼夜节律;转录组织;甲壳树果;脱像;心输出;

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专利

1. Molecular evidence for an intrinsic circadian pacemaker in the cardiac ganglion of the American lobster, Homarus americanus - Is diel cycling of heartbeat frequency controlled by a peripheral clock system ? [J] . Christie Andrew E., Yu Andy, Roncalli Vittoria, Marine genomics . 2018,第期

机译：在美国龙虾的心神经神经节中，仿木族的内在昼夜节目起搏器的分子证据 - 是由外围时钟系统控制的心跳频率的Diel循环吗？
2. Identification of a calcitonin-like diuretic hormone that functions as an intrinsic modulator of the American lobster, Homarus americanus, cardiac neuromuscular system [J] . Christie A. E., Stevens J. S., Bowers M. R., The Journal of Experimental Biology . 2010,第1期

机译：鉴定出降钙素样利尿激素，其起美洲龙虾，美洲大螯虾，心脏神经肌肉系统的内在调节剂的作用
3. Functional anatomy of the female reproductive system of the American lobster ( Homarus americanus Homarus americanus ) [J] . Comeau Michel, Benhalima Kadra Journal of Morphology . 2018,第11期

机译：美国龙虾女性生殖系统的功能解剖（Homarus Americanus Homarus Americanus）
4. Expression and Distribution of Neuropeptides in the Nervous System of the American Lobster Homarus americanus and Their Developmental Roles [C] . Ruibing Chen, Xiaoyue Jiang, Lingjun Li American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：美国龙虾仿性仿血症神经系统中神经肽的表达和分布及其发展作用
5. ELECTROPHYSIOLOGICAL STUDIES ON THE CARDIAC GANGLION OF THE LOBSTER, HOMARUS AMERICANUS, WITH EVIDENCE FOR THE EXISTENCE OF AN ELECTROGENIC SODIUM PUMP [D] . LIVENGOOD, DAVID ROBERT -1

机译：龙虾心球节的电生理学研究表明存在电致钠泵
6. Identification of a calcitonin-like diuretic hormone that functions as an intrinsic modulator of the American lobster Homarus americanus cardiac neuromuscular system [O] . A. E. Christie, J. S. Stevens, M. R. Bowers, -1

机译：鉴定出降钙素样利尿激素其起美洲龙虾美洲大螯虾心脏神经肌肉系统的内在调节剂的作用
7. Identification of a calcitonin-like diuretic hormone that functions as an intrinsic modulator of the American lobster, Homarus americanus, cardiac neuromuscular system [O] . Christie, A. E., Stevens, J. S., Bowers, M. R., 2009

机译：鉴定出降钙素样利尿激素，其起美洲龙虾，美洲大螯虾，心脏神经肌肉系统的内在调节剂的作用

Molecular evidence for an intrinsic circadian pacemaker in the cardiac ganglion of the American lobster, Homarus americanus - Is diel cycling of heartbeat frequency controlled by a peripheral clock system ?

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