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首页> 外文期刊>Molecular & cellular proteomics: MCP >Quantitative Protein Profiling of Chlamydia trachomatis Growth Forms Reveals Defense Strategies Against Tryptophan Starvation
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Quantitative Protein Profiling of Chlamydia trachomatis Growth Forms Reveals Defense Strategies Against Tryptophan Starvation

机译：衣原体衣原体的定量蛋白质谱分析增长形式揭示了对色氨酸饥饿的防御策略

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摘要

Chlamydia trachomatis is one of the most common sexually transmitted bacterial pathogens in humans. The infection is often asymptomatic and can lead to chronic manifestations. The infectious elementary body and the replicating reticulate body are the two growth forms in the normal developmental cycle. Under the influence of interferon-, the normal cycle is disrupted because of tryptophan degradation, leading to a third persistent form, the aberrant reticulate body. For the genital strain C. trachomatis D/UW-3/CX we established a quantitative, label-free proteomic approach, and identified in total 655 out of 903 (73%) predicted proteins, allowing the first quantitative comparison of all three growth forms. Inclusion membrane proteins and proteins involved in translation were more abundant in the reticulate body (RB)(1) and aberrant reticulate body (ARB) forms, whereas proteins of the type III Secretion System and the cell envelope were more abundant in the elementary body (EB) form, reflecting the need for these proteins to establish infection and for host interactions. In the interferon- induced ARB proteome, the tryptophan synthase subunits were identified as biomarkers with a strong increase from less than 0.05% to 9% of the total protein content, reflecting an inherent defense strategy for the pathogen to escape interferon- mediated immune pressure. Furthermore, the total tryptophan content in the ARB form was 1.9-fold lower compared with the EB form, and we demonstrate that modulation of the protein repertoire toward lower abundance of proteins with high tryptophan content, is a mechanism which contributes to establish and maintain chlamydial persistence. Thus, quantitative proteomics provides insights in the Chlamydia defense mechanisms to escape interferon- mediated immune pressure.
机译：Chlamydia Thachomatis是人类中最常见的性传播细菌病原体之一。感染往往是无症状的，可导致慢性表现。感染性小学体和复制网状体是正常发育循环中的两个生长形式。在干扰素的影响下，由于色氨酸降解，正常循环被破坏，导致第三持续形式，异常网状体。对于生殖器菌株C. Thachomatis D / UW-3 / CX，我们建立了无定量的无标记蛋白质组学方法，并以903（73％）预测的蛋白质中的总共655例鉴定，允许所有三种生长形式的第一次定量比较。掺入翻译的包涵体蛋白质和蛋白质在网状体（Rb）（1）（1）和异常网状体（Arb）形式中更丰富，而III型分泌系统的蛋白质和细胞包络在基本体内更丰富（ EB）形式，反映了这些蛋白质的需要建立感染和宿主相互作用。在干扰素诱导的Arb蛋蛋白组中，鉴定色氨酸合酶亚基被鉴定为生物标志物，其占蛋白质含量的小于0.05％至9％的生物标志物，反映了病原体的固有防御策略以逃避干扰介导的免疫压力。此外，与EB形式相比，ARB形式中的总色氨酸含量为1.9倍，我们证明蛋白质曲目的调节朝着较低的色氨酸含量较低的蛋白质，是一种有助于建立和维持衣原体的机制坚持。因此，定量蛋白质组学提供衣原体防御机制的见解，以逃避干扰介导的免疫压力。

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  • 来源
    《Molecular & cellular proteomics: MCP》 |2016年第12期|共11页
  • 作者

    Ostergaard Ole; Follmann Frank; Olsen Anja W.; Heegaard Niels H.; Andersen Peter; Rosenkrands Ida;

  • 作者单位

    Statens Serum Inst Dept Autoimmunol &

    Biomarkers DK-2300 Copenhagen Denmark;

    Statens Serum Inst Dept Infect Dis Immunol 5 Artillerivej DK-2300 Copenhagen S Denmark;

    Statens Serum Inst Dept Infect Dis Immunol 5 Artillerivej DK-2300 Copenhagen S Denmark;

    Statens Serum Inst Dept Autoimmunol &

    Biomarkers DK-2300 Copenhagen Denmark;

    Statens Serum Inst Dept Infect Dis Immunol 5 Artillerivej DK-2300 Copenhagen S Denmark;

    Statens Serum Inst Dept Infect Dis Immunol 5 Artillerivej DK-2300 Copenhagen S Denmark;

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  • 正文语种 eng
  • 中图分类 生物化学;
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