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首页> 外文期刊>Molecular Immunology >IgE recognition of chimeric isoforms of the honeybee (Apis mellifera) venom allergen Api m 10 evaluated by protein array technology
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IgE recognition of chimeric isoforms of the honeybee (Apis mellifera) venom allergen Api m 10 evaluated by protein array technology

机译：IgE识别蜂窝（API Mellifera）毒液过敏原API M 10的蜂窝状同种型（Apis Mellifera）评估蛋白阵列技术

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摘要

Api m 10 has recently been established as novel major allergen that is recognized by more than 60% of honeybee venom (HBV) allergic patients. Previous studies suggest Api m 10 protein heterogeneity which may have implications for diagnosis and immunotherapy of HBV allergy. In the present study, RT-PCR revealed the expression of at least nine additional Api m 10 transcript isoforms by the venom glands. Two distinct mechanisms are responsible for the generation of these isoforms: while the previously known variant 2 is produced by an alternative splicing event, novel identified isoforms are intragenic chimeric transcripts. To the best of our knowledge, this is the first report of the identification of chimeric transcripts generated by the honeybee. By a retrospective proteomic analysis we found evidence for the presence of several of these isoforms in the venom proteome. Additionally, we analyzed IgE reactivity to different isoforms by protein array technology using sera from HBV allergic patients, which revealed that IgE recognition of Api m 10 is both isoform- and patient-specific. While it was previously demonstrated that the majority of HBV allergic patients display IgE reactivity to variant 2, our study also shows that some patients lacking IgE antibodies for variant 2 display IgE reactivity to two of the novel identified Api m 10 variants, i.e. variants 3 and 4.
机译：最近已建立为新的主要过敏原，以超过60％的蜜毒（HBV）过敏患者认可的新型过敏原。以前的研究表明API M 10蛋白质异质性，这可能对HBV过敏的诊断和免疫治疗有影响。在本研究中，RT-PCR揭示了毒腺的至少九种另外的API M 10转录物同种型的表达。两个不同的机制负责产生这些同种型：而先前已知的变型2由替代剪接事件产生，新颖的鉴定同种型是腺体嵌合转录物。据我们所知，这是蜜蜂产生的嵌合成绩单鉴定的第一个报告。通过回顾性蛋白质组学分析，我们发现毒液蛋白质组中存在几种同种型的证据。此外，我们使用来自HBV过敏患者的血清分析了IgE反应对不同同种型的不同同种型，这揭示了API M 10的IgE识别是同种型和患者特异性。虽然前面表明大多数HBV过敏患者对变体2显示IgE反应性，但我们的研究还表明，一些缺乏用于变体2的IgE抗体的患者显示IgE反应性与两个新颖的鉴定API M 10变体，即变体3和4.

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  • 来源
    《Molecular Immunology》 |2014年第2期|共7页
  • 作者

    VanVaerenberghM.; DeSmetL.; Rafei-ShamsabadiD.; BlankS.; SpillnerE.; EboD.G.; DevreeseB.; JakobT.; deGraafD.C.;

  • 作者单位

    Laboratory of Molecular Entomology and Bee Pathology Ghent University Krijgslaan 281 S2Ghent;

    Laboratory of Molecular Entomology and Bee Pathology Ghent University Krijgslaan 281 S2Ghent;

    Allergy Research Group Department of Dermatology University Medical Center Freiburg Hauptstrasse;

    Center of Allergy and Environment (ZAUM) Technical University and Helmholtz Center Munich Ingolst;

    Department of Engineering Aarhus University Gustav Wieds Vej 10Aarhus C Denmark;

    Department of Immunology Allergology and Rheumatology University of Antwerp Universiteitsplein;

    Laboratory of Protein Biochemistry and Biomolecular Engineering Ghent University K.L;

    Allergy Research Group Department of Dermatology University Medical Center Freiburg Hauptstrasse;

    Laboratory of Molecular Entomology and Bee Pathology Ghent University Krijgslaan 281 S2Ghent;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    Allergy; Chimeric transcripts; Honeybee; Protein array; Venom;

    机译：过敏;嵌合成绩单;蜜蜂;蛋白质阵列;毒液;

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