A'/> Biocompatibility of hydroxyapatite coatings deposited by pulse electrodeposition technique on the Nitinol superelastic alloy
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Biocompatibility of hydroxyapatite coatings deposited by pulse electrodeposition technique on the Nitinol superelastic alloy

机译：脉冲电沉积技术在镍钛合金超弹性合金上沉积的羟基磷灰石涂层的生物相容性

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Abstract The present study deals with pulse electrochemical deposition of HA on NiTi alloy and in vitro evaluation of coatings. At first step, a thermo-chemical surface modification process was applied to control the Ni release of the alloy. The electrochemical deposition of CaP coatings was examined at both dilute and concentrated solutions. The morphology and the composition of coatings were studied using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Plate like and needle like morphologies were formed for dilute and concentrated solution respectively and HA phase was formed by increasing the pulse current density for both electrolyte. The thickness of the samples was measured using cross sectioning technique. Fibroblast cell culture test on the coated samples revealed that the HA coating obtained by dilute solution shows the best biocompatibility. Also, MTT assay showed the highest cell density and cell proliferation after 5days for the HA coating of dilute solution. The contact angle of samples was measured and the coated samples showed a hydrophilic surface. Soaking the sample in SBF revealed that the crystallization rate of calcium-phosphate compounds is higher on the plate like HA coating as compared to the needle like morphology. The P release of the HA coated samples was measured in a physiological saline solution and the results show that the ions releasing in the plate like coating are less than the needle like coating. It seems that the stability of the plate like coating in biological environments is responsible for the better biocompatibility of the coat
机译：<！[cdata [ 抽象 本研究涉及HA对Niti合金的脉冲电化学沉积及涂层的体外评价。在第一步，施加热化学表面改性方法以控制合金的Ni释放。 Ca P涂层的电化学沉积在稀释和浓缩的溶液中检查。使用扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）研究了涂料的形态和组成。形成形态的板状和针状物，分别形成稀释且浓缩溶液，通过增加电解质的脉冲电流密度形成HA相。使用横截面技术测量样品的厚度。涂覆样品上的成纤维细胞培养试验显示通过稀释溶液获得的HA涂层显示出最佳的生物相容性。此外，MTT测定显示出稀释溶液的HA涂层的5 天后的最高细胞密度和细胞增殖。测量样品的接触角，并涂覆的样品显示亲水表面。在SBF中浸泡样品显示，与像形态相比，钙磷酸钙化合物的结晶速率较高，如HA涂层相比。在生理盐水溶液中测量HA涂覆的样品的P释放，结果表明，在板上涂覆在板中的离子小于针涂层。似乎在生物环境中涂层的板的稳定性是涂层的更好的生物相容性

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