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首页> 外文期刊>Materials science & engineering, C. Materials for Biogical applications >Accelerated wound healing and its promoting effects of biomimetic collagen matrices with siderophore loaded gelatin microspheres in tissue engineering
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Accelerated wound healing and its promoting effects of biomimetic collagen matrices with siderophore loaded gelatin microspheres in tissue engineering

机译：造型胶原蛋白基质促进伤口愈合及其促进组织工程中的明胶微球的促进作用

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摘要

The prolonged inflammation and elevation of Matrix Metalloproteniases (MMPs) at the wound site causes significant degradation of Extracellular matrix (ECM) which cause delays the process of wound healing. Hence the development of therapeutic dressing matrices to control and to positively regulate MMPs balance was considered important in achieving faster healing. The design of biomaterial matrices of collagen scaffold has the challenge to mimic the function of ECM and emulate to the attraction of fibroblast migration at wound site. Herein, we report the fabricated Collagen (COL) matrices impregnated with Siderophore loaded Gelatin Microspheres (SGM) as a delivery system to control both infection and protease levels in the wound site for accelerated healing. The fabricated collagen scaffold impregnated with siderophore loaded gelatin microspheres (COL-SGM) was characterized physiochemically using Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) and swelling behaviour. The COL-SGM scaffold possesses good swelling ability and also exhibited better morphology for the cell adhesion and proliferation. The in vitro biocompatibility and in vitro fluorescence activity of the developed scaffold revealed to possess good cell proliferation and migration against NTH 3T3 fibroblast and Human keratinocytes (HaCaT) cell lines. Furthermore, the in vivo evaluation offered the advantage of neutralizing the excessive proteases and delivered the siderophore in controlled fashion depending on the level of wound exudates with modulated MMPs. Moreover, the COL-SGM scaffold exhibited with increase in the collagen synthesis and faster reepitheliazation of wounds. Thus the developed COL-SGM scaffold achieved improvements in biocompatibility and act as a potent MMP inhibitor to improve wound healing efficiency in tissue engineering application.
机译：伤口位点的延长炎症和基质金属蛋白（MMP）的升高导致细胞外基质（ECM）的显着降解，这导致造成伤口愈合过程。因此，在实现更快的愈合方面认为治疗敷料矩阵和对MMP平衡的持续调节MMP平衡的发展是重要的。胶原蛋白支架的生物材料基质的设计具有模拟ECM功能的挑战，并模拟伤口位点的成纤维细胞迁移的吸引力。在此，我们将制造的胶原（COL）基质报告为浸渍的膀胱管载着明胶微球（SGM）作为输送系统，以控制伤口部位的感染和蛋白酶水平以加速愈合。使用傅里叶变换红外光谱（FTIR），扫描电子显微镜（SEM）和溶胀行为，用傅里叶变换红外光谱（COL-SGM）浸渍具有膀胱载加载的明胶微球（COL-SGM）的制造的胶原蛋白。 COL-SGM支架具有良好的溶胀能力，并且还具有更好的细胞粘附和增殖形态。发育支架的体外生物相容性和体外荧光活性显示出具有良好的细胞增殖和对Nth 3T3成纤维细胞和人角蛋白细胞（HACAT）细胞系的迁移。此外，体内评估提供了中和过量蛋白酶的优点，并根据具有调节MMP的伤口水平的伤口水平，以受控方式递送纵向。此外，COL-SGM支架随着胶原蛋白合成的增加和伤口的速度较快。因此，发育的COL-SGM支架实现了生物相容性的改善，并充当有效的MMP抑制剂，以改善组织工程应用中的伤口愈合效率。

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