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首页> 外文期刊>LWT-Food Science & Technology >Optimization of fermentation medium for a newly isolated yeast strain ( Zygosaccharomyces rouxii JM-C46) and evaluation of factors affecting biosynthesis of D-arabitol
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Optimization of fermentation medium for a newly isolated yeast strain ( Zygosaccharomyces rouxii JM-C46) and evaluation of factors affecting biosynthesis of D-arabitol

机译：用于新分离的酵母菌菌株发酵培养基的优化（ rouxii JM-C46）和影响D- Arabitol生物合成的因素评估

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摘要

A newly isolated yeast strain, namelyZygosaccharomyces rouxiiJM-C46, was reported to be promising for enhancedD-arabitol production from glucose. In this study, further efforts were made to optimize fermentation conditions for this strain through one-factor-at-a-time strategy considering seven important factors of shake flask fermentation. Subsequently, composition of fermentation medium was optimized using Box-Behnken design (BBD) of response surface methodology (RSM) including three major nitrogen providing ingredients, namely yeast extract, (NH4)2SO4and peptone. Maximum D-arabitol concentration produced in one-factor-at-a-time experiments was found to be 72.69?g/L under the optimum conditions consisting of 200?g/L initial glucose, 100?ml medium, 5% (equivalent to 8.78?g/L) initial inoculum, temperature 30?°C, pH 5.0, fermentation time 96?h, and agitation rate 200?rpm. In the experiments of BBD, the predicted yield of D-arabitol varied between 67.73?g/L and 76.76?g/L, while the actual concentration of D-arabitol ranged from 68.02?g/L to 76.95?g/L. The optimum composition of medium F (final medium) containedd-glucose (200?g/L), yeast extract (10?g/L), (NH4)2SO4(2?g/L) and peptone (7.5?g/L), which further increased D-arabitol production from 72.69?g/L to 76.32?g/L.
机译：据报道，一种新孤立的酵母菌株Namelyzygosaccharomyces Rouxiijm-C46，对葡萄糖产生了增强的阿拉伯醇生产。在这项研究中，考虑到摇动瓶发酵的七种重要因素，通过一次因素 - 在一次性策略来优化这种菌株的发酵条件进一步努力。随后，使用响应面方法（RSM）的Box-Behnken设计（BBD）优化了发酵培养基的组成，包括三个主要氮提供成分，即酵母提取物，（NH4）2SO4和蛋白胨。在一次性实验中产生的最大D- arabitol浓度为72.69μm≤g/ l，其最佳条件包括200μm≤g/ l初始葡萄糖，100μl培养基，5％（相当于8.78？g / l）初始接种物，温度30≤℃，pH 5.0，发酵时间96Ω·h，搅拌率200？rpm。在BBD的实验中，D-阿拉伯醇的预测产率在67.73Ω·克/ L和76.76？G / L之间变化，而D-阿拉伯醇的实际浓度范围为68.02Ω·克/升至76.95？G / L。培养基F（最终介质）的最佳组合物含有 - 葡萄糖（200μl），酵母提取物（10〜G / L），（NH 4）2SO4（2→G / L）和蛋白胨（7.5？G / L. ），其进一步增加了72.69〜72.69〜76.32的D-阿拉伯醇生产。

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