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Sediment methane dynamics along the Elbe River

机译：沿易北河沉积物甲烷动力学

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Methane (CH4) is an important atmospheric trace gas mostly released from wet anoxic soils and sediments. While many studies have focused on relatively homogenous environments like rice fields and lake sediments, the changing contribution of heterogeneous sediments e.g. along the longitudinal profile of a rivers has not been covered very frequently. Here we investigated sediment samples from 11 locations of the Elbe River. Sediments were incubated to measure methanogenic/methanotrophic potentials and contribution of individual methanogenic pathways using isotope analysis of delta C-13. Additionally, we determined the diversity of the methanogenic communities (analysis of T-RFLP targeting the mcr-A gene in the sediment samples), while abundances of archaea, methanogens and methanotrophs were determined by qPCR. The CH4 production was detected in six samples (out of 11 examined) and ranged from 0.12 to 644.72 nmol gDW(-1) d(-1). Methanotrophy was found in all examined sediment samples and ranged from 654 to 10,875 nmol gDW(-1) d(-1). Abundance of methanogens and methanotrophs (Mcr-A and pmo-A gene copy numbers) was not significantly different and quite stable around 10(6) to 10(7) copies gDW(-1). The group specific qPCR showed high fluctuations, while the highest counts were reported for Methanomicrobiales and Methanosarcinales (10(5) to 10(8) copies per gram dry sediment), followed by Methanobacteriales (10(3) to 10(5) copies per gram dry sediment). A significant proportion of unidentified methanogens was found in almost every locality. Isotope analysis of delta C-13 showed that (CH4) is produced mainly by hydrogenotrophic methanogens. We see no trend in the studied parameters along the Elbe River. The molecular data showed no spatial characteristics, while we found hotspots of the measured CH4 processes (CH4 production and oxidation) due to other local driving factors (e.g. carbon content). Thus, out results indicate that the observed variability of the CH4 production and oxidation rates is only indirectly linked to the presence or quantities of different microbial guilds.
机译：甲烷（CH4）是一种重要的大气痕量气体，主要来自湿缺氧土壤和沉积物。虽然许多研究专注于稻田和湖泊沉积物等相对均匀的环境，但是异构沉积物的变化贡献。沿着河流的纵向轮廓尚未经常覆盖。在这里，我们研究了来自易北河11个地点的沉积物样本。孵育沉积物以测量δC-13同位素分析的同位素分析的单个甲状腺学途径的甲烷/甲基营养潜力和贡献。另外，我们确定了甲基甲基社区的多样性（靶向MCR-A基因的T-RFLP分析沉积物样品），而QPCR测定了大量的古痤疮，甲烷和甲蛋白酶。在六个样品中检测到CH 4生产（在11中的11个中），范围为0.12至644.72 nmol GDW（-1）D（-1）。在所有检查的沉积物样品中发现了甲基萎缩，范围为654至10,875 nmol GDW（-1）D（-1）。丰富的甲烷（MCR-A和PMO-A基因拷贝数）没有显着差异，左右10（6）至10（7）份GDW（-1）。本群特异性QPCR显示出高波动，而甲烷癌和甲烷癌癌（10（5）至10（8）个拷贝每克干沉淀物）报告的最高计数，其次是每克（10（3）至10（5）份/每份）克干燥沉积物）。在几乎每个地方都发现了一部分大量的未识别的甲烷。 Delta C-13的同位素分析表明（CH 4）主要由氢脱氢甲烷基因产生。我们在沿易北河沿着研究的参数看不到趋势。分子数据显示出不存在的空间特性，而我们发现由于其他局部驱动因子（例如碳含量）的其他局部驱动因子（例如碳含量）发现测量的CH4工艺（CH4生产和氧化）的热点。因此，输出结果表明，CH 4产生和氧化率的观察到可变性仅间接地与不同微生物公会的存在或数量相连。

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