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首页> 外文期刊>Renewable Agriculture and Food Systems >CbbR and RegA regulate cbb operon transcription in Ralstonia eutropha H16
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CbbR and RegA regulate cbb operon transcription in Ralstonia eutropha H16

机译：CBBR和Rega调节Ralstonia Eutropha H16中的CBB操纵子转录

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摘要

The biotechnologically important Gram-negative beta-proteobacterium Ralstonia eutropha H16 is able to grow lithoautotrophically by utilizing CO2 and H-2 as sole carbon and energy sources, respectively. CO2 is fixed by the CBB cycle, which is encoded in duplicate on the genome of R. eutropha H16. The transcription of both cbb operons is controlled by the transcription regulator CbbR dependent on intracellular PEP levels as a response to the carbon-state of the cell. As demonstrated in this study transcription control of both cbb operons appears to be more complex and additionally involves, next to CbbR, the transcription regulator RegA as part of the global transcription regulation system RegA/RegB. The identification of a highly conserved RegA/RegB homologue in R. eutropha H16 and experimental evidence gathered in this study reveal that RegA plays a crucial role in the transcription control of both cbb promoters. RegA is able to induce cbb promoter activity and controls transcription in combination with CbbR dependent on cellular PEP concentrations. These results clearly demonstrate that RegA plays an important role in cbb operon transcription regulation and may also be relevant for the control of other energy-utilizing and energy-generating pathways of R. eutropha H16. In addition to promoting a more complete understanding of the CO2 fixation mechanism of R. eutropha H16 these findings also provide crucial insights for the utilization of this bacterium in biotechnological applications with respect to CO2 fixation.
机译：生物技术重要的革兰氏阴性β-蛋白杆菌蛋白质聚苯杆菌eutropha H16分别能够分别使用CO 2和H-2作为唯一碳和能量来生长繁律上。 CO 2通过CBB循环固定，其在R. Eutropha H16的基因组上重复编码。 CBB操纵子的转录由转录调节剂CBBR控制，依赖于细胞内PEP水平作为对细胞的碳态的反应。如本研究中所示，两个CBB操纵子的转录控制似乎更复杂，另外涉及CBBR，转录调节器Rega作为全局转录调节系统Rega / Regb的一部分。鉴定R. eutropha H16中高度保守的Rega / Regb同源物和本研究中聚集的实验证据表明，Rega在两种CBB启动子的转录控制中起着至关重要的作用。 Rega能够诱导CBB启动子活性，并与依赖于细胞PEP浓度的CBBR组合进行转录。这些结果清楚地表明，Rega在CBB操纵子转录规则中发挥着重要作用，也可能与控制R. eutropha H16的其他能量利用和能量产生途径有关。除了促进R. Eutropha H16的CO2固定机制外，这些发现还提供了在生物技术应用中利用这种细菌的关键见解，相对于CO 2固定。

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