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首页> 外文期刊>Renewable Agriculture and Food Systems >Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation
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Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation

机译：全细胞反应和产物分离的整合：高度疏水性溶剂促进原位衬底供应，简化萃取产品分离

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Product isolation from aqueous-organic reaction mixtures that contain high concentrations of whole cells constitutes a challenging task in bioprocessing. Stirring of the biphasic reaction media leads to the formation of solvent droplets coated by cells and other surface active components and an emulsion forms. We used an early focus on phase separation to simplify a whole-cell bioreduction. Octanol, heptanol, hexanol, hexane and dipropylether were tested as co-solvents in the E. coli catalyzed reduction of o-chloroacetophenone. All solvents showed very similar performance in bioreductions and highest yields were obtained with low organic-to-aqueous phase ratios. Reaction mixtures were directly investigated for organic-phase recovery. Phase separation was optimized in small-scale settling experiments and confirmed by the isolation of 20.4 g (S)-1-(2-chlorophenyl)ethanol from a 0.5 L batch reduction containing 40 g(CDW)/L whole-cell catalyst. Solvent consumption during product isolation could be halved by the simple addition of sodium hydroxide prior to product extraction. Basification to pH 13.5 and three extraction steps with a total of 1.2 v/v hexane led to an isolated yield of 87% (97% reduction yield). A general emulsion destabilizing effect under harsh conditions, as extreme pH values and presence of toxic reactants, was observed. (C) 2016 The Authors. Published by Elsevier B.V.
机译：来自含有高浓度全细胞的水性反应混合物的产品分离构成了生物处理的挑战性任务。双相反应介质的搅拌导致形成由细胞和其他表面活性组分和乳液形式的涂覆的溶剂液滴。我们使用早期重点关注相分离，以简化全细胞生物保险。在大肠杆菌催化还原O-氯代乙酮的大肠杆菌中，测试辛醇，庚醇，六醇，己烷和双丙醚。所有溶剂在生物调节中表现出非常相似的性能，并以低有机 - 水相比获得最高产率。直接研究反应混合物以进行有机相回收。在小规模沉降实验中优化相分离，并通过从含有40g（CdW）/ L全细胞催化剂的0.5L分批还原的0.4g（S）-1-（2-氯苯基）乙醇的分离证实。产物分离过程中的溶剂消耗可以通过在产物提取之前简单地加入氢氧化钠。碱化至pH13.5和总共1.2V / v己烷的三个提取步骤导致分离的产率为87％（减少97％）。观察到苛刻条件下的一般乳液不稳定效果，作为极端pH值和有毒反应物的存在。 （c）2016年作者。 elsevier b.v出版。

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