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首页> 外文期刊>Parasitology >Tools for diagnosis, monitoring and screening of Schistosoma infections utilizing lateral-flow based assays and upconverting phosphor labels.
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Tools for diagnosis, monitoring and screening of Schistosoma infections utilizing lateral-flow based assays and upconverting phosphor labels.

机译：利用横向流动的测定和上变磷光体标记的诊断，监测和筛查血吸虫瘤感染的工具。

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The potential of various quantitative lateral flow (LF) based assays utilizing up-converting phosphor (UCP) reporters for the diagnosis of schistosomiasis is reviewed including recent developments. Active infections are demonstrated by screening for the presence of regurgitated worm antigens (genus specific polysaccharides), whereas anti-Schistosoma antibodies may indicate ongoing as well as past infections. The circulating anodic antigen (CAA) in serum or urine (and potentially also saliva) is identified as the marker that may allow detection of single-worm infections. Quantitation of antigen levels is a reliable method to study effects of drug administration, worm burden and anti-fecundity mechanisms. Moreover, the ratio of CAA and circulating cathodic antigen (CCA) is postulated to facilitate identification of either Schistosoma mansoni or Schistosoma haematobium infections. The UCP-LF assays allow simultaneous detection of multiple targets on a single strip, a valuable feature for antibody detection assays. Although antibody detection in endemic regions is not a useful tool to diagnose active infections, it gains potential when the ratio of different classes of antibody specific for the parasite/disease can be determined. The UCP-LF antibody assay format allows this type of multiplexing, including testing a linear array of up to 20 different targets. Multiple test spots would allow detection of specific antibodies, e.g. against different Schistosoma species or other pathogens as soil-transmitted helminths. Concluding, the different UCP-LF based assays for diagnosis of schistosomiasis provide a collection of tests with relatively low complexity and high sensitivity, covering the full range of diagnostics needed in control programmes for mapping, screening and monitoring.
机译：利用上转换荧光体（UCP）报告者进行用于诊断血吸虫病的各种定量横向流动（LF）测定的潜力是综述，包括最近的发展。通过筛选反流蠕虫抗原（特异性多糖）的存在来证明活性感染，而抗血吸虫抗体可能表明正在进行的和过去的感染。血清或尿液中的循环阳极抗原（CAA）被鉴定为可允许检测单蠕虫感染的标志物。抗原水平的定量是研究药物管理，蠕虫负荷和抗融合机制的可靠方法。此外，假设CAA和循环阴极抗原（CCA）的比例以促进血吸虫麦森或血吸虫血吸虫感染的鉴定。 UCP-LF测定允许同时检测单条带上的多个靶，是抗体检测测定的有价值的特征。虽然流行区域中的抗体检测不是诊断有源感染的有用工具，但是当可以确定对寄生虫/疾病的不同类别的抗体的比率来增益。 UCP-LF抗体测定格式允许这种类型的多路复用，包括测试最多20个不同目标的线性阵列。多个测试点允许检测特异性抗体，例如特异性抗体。针对不同的血吸虫瘤或其他病原体作为土壤传播的蠕虫。结论，血吸虫病诊断的不同UCP-LF测定提供了一系列具有相对较低的复杂性和高灵敏度的测试，涵盖了控制程序所需的全部诊断，用于映射，筛选和监控。

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