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首页> 外文期刊>Pharmacology, Biochemistry and Behavior >Response to ethanol induced ataxia between C57BL/6J and 129X1/SvJ mouse strains using a treadmill based assay
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Response to ethanol induced ataxia between C57BL/6J and 129X1/SvJ mouse strains using a treadmill based assay

机译：使用基于跑步机的测定法对C57BL / 6J和129x1 / SVJ小鼠菌株之间的乙醇诱导共济失调

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More sensitive assays of mouse motor ataxia may provide a better understanding of the pathological profile. Treadmill gait analysis using ventral imaging allows for unhindered access to the ambulating mouse. In contrast to genetic mutations or exogenous brain injury, ethanol (EtOH) allows for the detection of dose dependent changes in motor behavior, which can be used to assess an assay's detection sensitivity. EtOH induced ataxia was assessed in C57BL/6J (B6) and 129X1/SvJ (129) mice using the DigiGait imaging system. Gait was analyzed across EtOH dosage (1.75, 2.25 and 2.75 g/kg) in each strain using a linear mixed effects model. Overall, 129 mice displayed greater susceptibility to EtOH ataxia than their B6 counterparts. In both strains, hind paws exhibited greater sensitivity to EtOH dosage than fore paws. Across most variables analyzed, only a modest EtOH-induced change in motor behavior was observed in each strain with the 1.75 g/kg EtOH doses failing to elicit significant change. These data indicate the ability to detect motor differences between strains, yet only moderate ability to detect change across EtOH dosage using the automated treadmill. Rotarod assays, however, were able to detect motor impairment at lower doses of EtOH. The significant, but opposite changes in paw placement with increasing EtOH doses highlight strain-specific differences in biophysical adaptations in response to acute EtOH intoxication. ? 2012 Elsevier Inc. All rights reserved.
机译：小鼠电机共济失纲目的更敏感的测定可以更好地了解病理概况。使用腹侧成像的跑步机步态分析允许无阻碍地进入雄心鼠标。与遗传突变或外源性脑损伤相比，乙醇（EtOH）允许检测电动机行为的剂量依赖性变化，其可用于评估测定的检测灵敏度。使用Digigait成像系统，在C57BL / 6J（B6）和129x1 / SVJ（129）小鼠中评估EtOH诱导的共济失调。使用线性混合效应模型，在每个菌株中通过EtOH剂量（1.75,2.25和2.75g / kg）分析步态。总体而言，129只小鼠对Etoh Ataxia的易感性显示于比其B6对应物更大。在两个菌株中，后爪对EtOH用量表现出比前爪的更高敏感性。在大多数分析的变量中，在每个菌株中只观察到运动行为的适度诱导的电动机行为变化，1.75g / kg eTOH剂量未能引起显着变化。这些数据表明能够检测菌株之间的电动机差异，但仅使用自动跑步机检测eTOH剂量的变化的中等能力。然而，Rotarod测定能够以较低剂量的EtOH检测电动机损伤。随着EtOH的增加，爪子放置的重要性但相反的变化突出了急性EtOH中毒的生物物理适应的菌株特异性差异。还2012年elsevier Inc.保留所有权利。

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